PRECLINICAL MOUSE MODELS: CENTRAL NERVOUS SYSTEM CANCERS

临床前小鼠模型：中枢神经系统癌症

• 批准号: 7957608
• 负责人: TERRY A VAN DYKE
• 金额: $ 4.68万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-15 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957608
• 关键词: Address; Area; Astrocytes; Astrocytoma; Brain; Computer Retrieval of Information on Scientific Projects Database; Funding; Glioma; Grant; Human; Image Analysis; Institution; Knock-out; Lesion; Malignant Neoplasms; Malignant neoplasm of central nervous system; Maps; Modeling; Molecular; Morphology; Mus; Pathway interactions; Primary Brain Neoplasms; Property; Protein p53; Publications; Research; Research Personnel; Resources; Source; Techniques; Transgenic Organisms; United States National Institutes of Health; design; molecular pathology; mouse model; pre-clinical; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Astrocytic glioma is the most prevalent primary brain tumor in human. Rarely responding to standard tumor therapies, it is one of the most deadly human tumors. The Van Dyke lab recently developed trangenic mouse model for astrocytoma by inactivating the Rb pathway, which occurred in 90% of the human counterpart. This model closely resembles the pathology and molecular pathways of human glioma (see publication). Little is understood about the properties of astrocytes elicited by cancer-promoting lesions in distinct brain microenvironments either in human cases or in our model. The aim of this project is designed to address distinct questions about the morphology, distribution and molecular constituents associated with normal,and tumor-associated astrocytes. We propose to use the techniques devloped in NCMIR to investigate 1 tumor-associated and peritumoral astrocytic morphology, 2 astrocytic distribution and boundaries within tumors and 3 differences in distribution of molecular constituents associated with tumor and normal surrounding areas by large scale mapping. The PI, Dr. Terry Van Dyke has utilized mouse manipulation strategies to study cancer for 16 years. In the course of analyzing the tumor suppressors p53 and pRb, her lab has established several tumor models using transgenic and knock-out strategies.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 星形细胞胶质瘤是人类最常见的原发性脑肿瘤。 它很少对标准肿瘤治疗有反应，是最致命的人类肿瘤之一。 货车戴克实验室最近开发了转基因小鼠星形细胞瘤模型，通过灭活Rb通路，这发生在90%的人类对应物中。 该模型与人类神经胶质瘤的病理学和分子途径非常相似（见出版物）。 无论是在人类病例中还是在我们的模型中，对不同脑微环境中的促癌病变引起的星形胶质细胞的性质都知之甚少。 该项目的目的是解决与正常和肿瘤相关的星形胶质细胞的形态，分布和分子组成有关的不同问题。 我们建议使用NCMR中开发的技术来研究1肿瘤相关和瘤周星形胶质细胞形态，2肿瘤内星形胶质细胞的分布和边界，以及3通过大尺度映射与肿瘤和正常周围区域相关的分子成分分布的差异。 PI，特里货车戴克博士已经利用小鼠操作策略研究癌症16年。在分析肿瘤抑制因子p53和pRb的过程中，她的实验室使用转基因和敲除策略建立了几种肿瘤模型。