Core--Imaging

成像核心设施将在光学显微镜成像和分析领域为该项目提供服务。该中心是围绕医学部肾内科最先进的设备而建立的，并受益于关键核心人员在成像方法应用于造血细胞功能分析方面的经验。该设施目前配备了两台共聚焦激光扫描显微镜：一台蔡司LSM 510能够紫外激发荧光；以及BioRad MRC-1024型双光子激发Ti：蓝宝石激光器和用于常规共聚焦成像的Kr/Ar激光器。两个宽视场显微镜架配备冷却CCD相机：应用精密三角电视系统优化图像反褶积；和第二个尼康台用于运动分析。具有CO2灌注的阶段孵化器可用于在显微镜阶段长时间维持和成像活细胞。可用的计算资源包括装有图像处理、图像反褶积、三维图像绘制和图像数据定量分析软件的工作站。成像核心的目的是提供1)通过透射光显微镜和荧光显微镜成像活造血细胞的技术和专业知识，以促进信号事件和Rac2依赖过程的实时分析；2)对荧光显微镜生成的图像数据进行定量分析的专业知识和支持，以补充造血细胞功能的生化和其他分析。

The Imaging Core facility will service the program in the area of light microscope imaging and analysis. The core is built around the state-of- the-art facility available in the Nephrology Division of the Department of Medicine, and benefits from the experience of key core personnel in the application of imaging methodologies to the analysis of hematopoietic cell function. The facility is currently equipped with two confocal laser-scanning microscopes: a Zeiss LSM 510 capable of UV excitation of fluorescence; and a BioRad MRC-1024 with Ti: sapphire laser capable of 2-photon excitation as well as Kr/Ar laser for conventional confocal imaging. Two wide-field microscope stands equipped with cooled CCD cameras are available: an Applied Precision Deltavision system optimized for image deconvolution; and a second Nikon stand used for motility assays. Stage incubators with CO2 perfusion are available for maintaining and imaging live cells on the stage of the microscope for long periods. Computing resources available include workstations with software for image processing, image deconvolution, rendering of 3-D image volumes and quantitative analysis of image data. The aim of the imaging core is to provide 1) technology and expertise in imaging live hematopoietic cells by transmission light microscopy and fluorescence microscopy to facilitate real-time analysis of signaling events and Rac2 dependent processes; and 2) expertise and support for quantitative analysis of image data generated by fluorescence microscopy to complement biochemical and other analysis of hematopoietic cell function.