Electrochemical Measurement of S-nitrosothiols

描述(申请人提供)：拟议的研究为体内和体外S-亚硝硫醇传感器的商业开发提供了一种创新的方法。将为广泛的生物应用设计选择性和灵敏的RSNO传感器和相关仪器。使用灵敏的一氧化氮电化学传感器与铜(I)离子发生器和输送系统相结合，证明了在纳摩尔范围内检测到RSNO。RSNO传感器将对低分子量(蛋白质)RSNO和高分子量(蛋白质)RSNO进行优先检测。本建议将致力于实现以下目标：(1)RSNO传感器，进一步与高效液相系统相结合，在线分离和检测不同的RSNO；(2)开发和商业化与RSNO传感器一起使用的电子控制仪器；(3)开发适用于生物采样的新型RSNO传感器；(4)RSNO检测器和仪器的生物现场测试。这些目标将通过重要的电分析、生物分析开发和详细的生物学研究来实现。这项拟议的研究将利用该小组在无纳米传感器、微芯片传感器以及新型微型混合器和铜(I)发生器方面的最新进展。拟议项目的长期目标是创造商业上可行和坚固耐用的仪器，用来快速、选择性和灵敏地测量S-亚硝硫醇的浓度，并能够在生物相关的浓度范围内检测这些物种。

DESCRIPTION (provided by applicant): The proposed research offers an innovative approach to the commercial development of in vivo and in vitro S-nitrosothiols (RSNO) sensors. The design of selective and sensitive RSNO sensors and related instrumentation will be undertaken for a wide range of biological applications. The use of sensitive nitric oxide electrochemical sensors coupled with a Cu(I) ion generator and delivery system, demonstrates detection of RSNOs in the nanomolar range. The RSNO sensors would offer preferential detection of both low and high molecular weight (protein) RSNOs. The following aims will be pursued in this proposal (1) the RSNO sensor, further combines with HPLC system to separate and detect different RSNOs on line 2) development and commercialization of an electronic controller instrument for use with RSNO sensors, (3) development of new RSNO sensors suitable for biological sampling (4) biological field testing of the RSNO detector and instrumentation. These goals will be reached with significant electroanalytical, bioanalytical development and detailed biological studies. The proposed research will capitalize upon the group's recent advances in NO nanosensors, microchip sensors, and a novel micromixer and Cu(I) generator. The long term goal of the proposed project is to create commercially viable and robust instrumentation with which to measure the concentration of S-nitrosothiols rapidly, selectively and with sensitivity capable of detecting these species in the biologically relevant concentration range.