LOW RESOLUTION VIRUS CRYSTALLOGRAPHY SOLUTION TO PHASE PROBLEM

最低分辨率反射和15 E之间的数据将是 从各种二十面体病毒的单晶中收集 外壳蛋白中产生的颗粒和病毒样颗粒 表达系统和改进的低角散射相机。 的 数据将用于生成20 E分辨率的3D结构， 二十面体调和分析 这些建筑将用于三个 目的 1. 用于与先前确定的比较，可比较的 分辨率，用低温电子图像分析产生的结构 显微镜数据，目的是建立对比质量 应用于EM数据的传递函数校正。 2. 使用 用于相位扩展至15 E分辨率的X射线衍生结构，或 以查看低分辨率X射线数据是否解决了相位 病毒晶体学的问题 3. 检查RNA或DNA密度 在所有病毒中的分布，以证明二十面体对称性 以及不同的电荷中和策略对 其组织。

Data between the lowest resolution reflection and 15 E will be collected from single crystals of a variety of icosahedral virus particles and virus-like particles generated in coat protein expression systems and a modified low angle scattering camera. The data will be used to generate 3D structures at 20 E resolution using icosahedral harmonic analysis. The structures will be used for three purposes. 1. For comparison with previously determined, comparable resolution, structures generate with image analysis of cryo electron microscopy data with the goal to establish the quality of the contrast transfer function corrections applied to the EM data. 2. Use the x-ray derived structures for phase extension to 15 E resolution or higher to see if the low resolution x-ray data solves the phase problem for virus crystallography. 3. Examine the RNA or DNA density distribution in all the viruses for evidence of icosahedral symmetry and for the influence of different charge neutralizing strategies on its organization.