Genetics of Anopheles funestus populations

描述（由申请方提供）：与冈比亚按蚊和 一个. arabiensis、阿拉伯隐翅虫An. funestus是世界上最重要的疟疾病媒之一， 世界在非洲部分地区，子孢子率高达9%， 明确这一点。在任何全面的控制计划中， 或者消灭疟疾无论控制策略是什么 针对安。冈比亚公司必须结合控制， 一个. funestus。因为目前不可能维持安的殖民地。 funestus在实验室里，既不是经典的遗传控制方案， 可以开发转基因策略。然而，详细的 对病媒种群结构的理解超越了遗传控制 方案，因为这方面的知识也是至关重要的成功使用 鉴于财政和人力资源有限，我们需要在病媒控制中使用杀虫剂。 这一知识允许对向量的响应进行预测 人类干预或遗传变化，例如，的潜力 导致杀虫剂抗药性的突变的传播。可惜 群体遗传结构对funestus的了解很少， 迄今为止相对未被研究过。很少有分子遗传标记 为这个物种而开发。本提案中概述的实验将 利用我们开发的细胞遗传学分子标记， 解决有关基因流内和之间的一个基本问题。不吉 人口。一个德谟的大小是多少，距离的影响是什么， 栖息地对族群间基因流动的影响携带的是替代染色体 反转安排生殖隔离？在流行病学上， 咬和休息行为的相关差异吗从以下来源获得的信息： 使用这些标记将使我们能够更有效地管理 目前可用的媒介控制工具，并将奠定必要的 如果遗传学是对抗An的策略的基础。funestus成为可能

DESCRIPTION (provided by the applicant): Together with Anopheles gambiae and An. arabiensis, An. funestus is among the most important malaria vectors in the world. With sporozoite rates as high as 9 percent in parts of Africa, it is clear that An. funestus cannot be ignored in any comprehensive plan to control or eradicate malaria from this continent. Whatever control strategy is implemented against An. gambiae s.l. must be integrated with control against An. funestus. Because it is currently impossible to maintain colonies of An. funestus in the laboratory, neither classical genetic control schemes nor transgenic strategies can be developed. However, the importance of a detailed understanding of vector population structure transcends genetic control programs, as this knowledge is also pivotal to the successful use of insecticides in vector control, given limited financial and human resources. This knowledge allows predictions to be made about the response of vector populations to human interventions or genetic changes, e.g., the potential for spread of mutations conferring insecticide resistance. Unfortunately, the population genetics structure of An. funestus is poorly understood and relatively unstudied to date. Very few molecular genetic markers have been developed for this species. The experiments outlined in this proposal will employ cytogenetically mapped molecular markers that we have developed to address fundamental questions about gene flow within and between An. funestus populations. What is the size of a deme and what is the effect of distance and habitat on gene flow between demes? Are carries of alternative chromosomal inversion arrangements reproductively isolated? Do they have epidemiologically relevant differences in biting and resting behavior? Information gained from the use of these markers will enable us to more efficiently manage the currently available tools for vector control and will lay the necessary foundation if genetis strategies against An. funestus become possible