POLYOMA T ANTIGEN FUNCTIONS IN CELL CULTURE

我们在这个项目中的工作是关于鉴定多瘤病毒中、小肿瘤抗原的离散功能，因为它们与培养中的病毒复制和细胞转化以及小鼠中肿瘤的复制和诱导有关。第一组的目标集中在中间T抗原作为主要的转化蛋白，其在结合磷脂酶C的作用，其作为细胞周期蛋白依赖性蛋白激酶的底物的可能的重要性，以及通过SH 3或其他细胞蛋白的信号转导蛋白的脯氨酸丰富的部分的可能的作用。第二组目标集中于小T蛋白，特别是在该蛋白表达中被阻断的病毒突变体的行为。将研究参与结合磷酸酶PP 2A或DNA-J结构域的特定序列的作用。该项目与计划项目资助的更大框架有关，该框架汇集了一组互动的研究人员，他们都关注研究含DNA肿瘤病毒的癌蛋白功能。

Our work in this project is concerned with identifying discrete functions of the polyoma virus middle and small tumor antigens as they relate to virus replication and transformation of cells in culture, and to replication and induction Of tumors in the mouse. The first group of Aims focuses on the middle T antigen as the major transforming protein, its role in binding phospholipase C, the possible importance of its serving as a substrate for a cyclin dependent protein kinase, and the possible role of a proline-rich segment of the protein in transducing signals via SH3 or other cellular proteins. The second group of Aims focuses on the small T protein and particularly on the behavior of a virus mutant blocked in expression of this protein. The role of specific sequences involved in binding the phosphatase PP2A, or the DNA-J domain, will be studied. This project relates to the larger framework of the Program Project Grant which brings together an interactive group of investigators all concerned with studying oncoprotein functions of DNA-containing tumor viruses.