Core--Primate facility

该项目的长期目标是通过将被动免疫与人中性单克隆抗体的协同组合与DNA引物/蛋白质增强策略的主动疫苗接种相结合，制定疫苗策略和免疫预防措施，以防止分娩时HIV亚型C的传播。该核心的总体目标是提供所需的实验动物和支持服务，以促进完成项目2（被动和主动免疫）和项目3 （DNA引物/蛋白质增强疫苗接种）中概述的目标。这将包括从耶基斯猕猴繁殖地提供新生和幼年无逆转录病毒（SIV/ STLV-1， SRV）恒河猴；口腔猕猴和HIV猴；用DNA或蛋白质免疫原对实验动物进行免疫；单克隆抗体单独使用或与主动免疫联合使用；免疫动物的病毒攻击；对实验动物进行日常监测；定期对实验动物进行身体检查，收集血液进行血象和免疫评估，并运送给医生。Ruprecht和Rasmussen的实验室进行体外研究；CBCs的表现和流式细胞术评估以确定淋巴细胞亚群；以及在研究过程中死亡或牺牲的所有实验动物的完整的大体和组织学评估。提供这些资源和支助服务将促进本方案项目中提出的艾滋病免疫战略的制定和试验。这些研究还将记录编码HIV分支C的环境基因的嵌合猿人免疫缺陷病毒的传染性和致病性，并将允许评估疫苗在预防感染（灭菌免疫）或修改攻毒后病毒载量方面的功效。

The long-range goal of this Program Project is to develop vaccine strategies and immunoprophylaxis against intrapartum transmission of HIV clade C by combining passive immunization with synergistic combinations of human neutralizing monoclonal antibodies with active vaccination with a DNA prime/protein boost strategy. The overall objectives for this Core are to provide the required experimental animals and support services needed to facilitate completion of the objectives outlined in Project 2 (Passive and Active Immunization) and Project 3 (DNA Prime/Protein Boost Vaccination). This will include provision of newborn and juvenile retrovirus-free (SIV/ STLV-1, SRV) rhesus macaques from the Yerkes macaque breeding colonies; oral and HIV macaques; immunizations of the experimental animals with DNA or protein immunogens; use of monoclonal antibodies alone or in combination with active immunization; viral challenge of the immunized animals; daily monitoring of the experimental animals; periodic physical examinations of the experimental animals and blood collections for hemogram and immunologic evaluations and for shipment to Drs. Ruprecht and Rasmussen's laboratories for in vitro studies; performance of CBCs and flow cytometry evaluations to determine lymphocyte subsets; and the performance of complete gross and histologic evaluation of all experimental animals that die or that are sacrificed during the course of the study. Provision of these resources and support services will facilitate the development and testing of the AIDS immunization strategies proposed in this Program Project. These studies will also document the infectivity and pathogenicity of the chimeric simian-human immunodeficiency virus encoding the env gene of HIV clade C, and will allow an assessment of vaccine efficacy with respect to either the prevention of infection (sterilizing immunity) or modification of post challenge virus load.