CORE--MASS SPECTROMETRY LABORATORY

描述(改编自应用程序)：质谱学成为 1968年范德比尔特大学研究景观的重要固定装置 当J·塞罗克·沃森博士加入教职员工并开始使用STRATE进行研究时 临床药理学中的同位素。1983年，伊恩·A·布莱尔博士应聘到 将质谱学重新确立为大学的核心设施。在一月份 1997年，布莱尔博士离开范德比尔特大学，在加州大学担任教职。 宾夕法尼亚州。劳伦斯·J·马内特博士承担了 在常任主任之前，质谱仪设施是临时的 可能会被招募。1998年2月，理查德·M·卡普里奥利博士加入 生物化学系的教职员工和他发起了重组步骤 并翻新设施，购置新的人员和仪器。前者 质谱学资源已重组为范德比尔特大学 质谱学研究中心(VU-MSRC)，如下所述。1998年5月， 大卫·L·哈奇博士加入了范德比尔特药理学学院，并假设 共用仪器设备的日常管理。卡普里奥利医生目前 为他的研究项目招募学生、博士后研究员和工作人员， 虽然哈奇博士正在招募更多的工作人员来重建共享的 仪器程序。VU-MSRC目前(1998年8月)有9名工作人员， 其中七人与共享仪器设施有关。翻新和 征聘新工作人员的工作将于1999年春季完成。 MSRC位于医学研究I号楼(MRBI)的8楼。这个 质谱学核心设施有七个质谱仪和三个高效液相 系统，质谱学研究设施有另外四个 乐器。可以对任何质量块进行结构分析 分光计。然而，三个三重四极杆仪器尤其是 擅长使用串联MS/MS技术进行结构测定 碰撞诱导解离。常规的定量分析是最多的 通过配备UV或电化学的高效液相系统经济地执行 探测器。然而，当出现更困难的分析分析时，质量 光谱分析提供了比简单的高效液相色谱更好的选择性和灵敏度，通常 具有飞摩尔或皮摩尔的检测极限。此外，当结构或 定量问题需要非常高的性能，新的高灵敏度 卡普里奥利博士的实验室可以通过以下两种方式获得微量分析技术 飞行时间或离子陷阱质谱计，配备有 提供阿托莫尔到飞托莫尔的纳米电喷雾电离源 敏感度。 质谱仪核心是范德比尔特大学弥撒的一部分 位于医学院的光谱研究中心(MSRC)。MSRC 由哈奇博士领导的核心质谱学服务实验室组成 以及卡普里奥利博士的研究实验室。除了提供例程之外 通过CNRU的核心设施提供分析和质谱分析服务 调查人员可以与卡普里奥利博士合作，解决分析和 需要非常规、尖端技术的结构性问题。作为较新版本 技术变得更好地被理解，它们被提供给用户 社区通过核心服务实验室。因此，MS Core满足 CNRU调查人员的常规分析需求，并提供新的 对需要更多创新的研究问题的分析能力 超出服务实验室范围的解决方案。

DESCRIPTION (adapted from the application): Mass Spectrometry became an important fixture of the research landscape at Vanderbilt University in 1968 when Dr. J. Throck Watson joined the faculty and initiated studies using stable isotopes in Clinical Pharmacology. In 1983 Dr. Ian A. Blair was recruited to reestablish mass spectrometry as a core facility for the university. In January 1997, Dr. Blair left Vanderbilt to take a faculty position at the University of Pennsylvania. Dr. Lawrence J. Marnett assumed administrative responsibility for the mass spectrometry facility on an interim basis until a permanent Director could be recruited. In February 1998, Dr. Richard M. Caprioli joined the faculty in the Department of Biochemistry and he initiated steps to reorganize and renovate the facilities, acquire new personnel and instruments. The former mass spectrometry resources have been restructured as the Vanderbilt University Mass Spectrometry Research Center (VU-MSRC), as described below. In May 1998, Dr. David L. Hachey joined the Vanderbilt Pharmacology faculty and assumed daily management of the shared instrument facility. Dr. Caprioli is currently recruiting students, postdoctoral fellows and staff for his research program, while Dr. Hachey is recruiting additional staff to rebuild the shared instrument program. The VU-MSRC currently (August 1998) has nine staff members, of whom seven are involved with the shared instrument facility. Renovations and recruitment of new staff will be complete by the spring of 1999. The MSRC is located on the 8th floor of Medical Research Building I (MRBI). The Mass Spectrometry Core facility has seven mass spectrometers and three HPLC systems, and the mass spectrometry research facility has four additional instruments. Structural analysis can be accomplished on any of the mass spectrometers. However, the three triple quadrupole instruments are especially adept at structural determination using tandem MS/MS techniques and collision-induced dissociation. Routine quantitative assays are most economically performed by HPLC systems equipped with UV or electrochemical detectors. However, when more difficult analytical assays arise, mass spectrometry affords better selectivity and sensitivity than simple HPLC, often with femtomole or picomole detection limits. Furthermore, when structural or quantitative problems demand the very highest performance, new high sensitivity microanalytical techniques are available in Dr. Caprioli's laboratory on either a time-of-flight or an ion trap mass spectrometer equipped with a nanoelectrospray ionization source that afford attomole to femtomole sensitivity. The Mass Spectrometry Core is part of the Vanderbilt University Mass Spectrometry Research Center (MSRC) located in the Medical School. The MSRC consists of a core mass spectrometry service laboratory directed by Dr. Hachey and the research laboratories of Dr. Caprioli. In addition to providing routine analytical and mass spectrometric services via the core facilities, CNRU investigators can form collaborations with Dr. Caprioli to solve analytical and structural problems that require non-routine, cutting edge technology. As newer techniques become better understood, they are made available to the user community through the core service laboratory. Thus, the MS Core satisfies the routine analytical needs of CNRU investigators, and also makes available new analytical capabilities for research problems demanding more innovative solutions that are beyond the scope of a service laboratory.