Novel, sustainable approaches for controlling fungal pathogens

该项目将调查天然抗真菌抑制剂，并评估与其他药物的协同/拮抗相互作用，扩展到作用模式表征以及环境或介质类型对相互作用的影响。这将包括测试，以寻找耐药真菌分离株可能对其过敏的药物，例如，耐药白念珠菌菌株的“附带敏感性”。此外，该项目将研究真菌抗附着聚合物作为一种新型的被动手段来控制有问题的真菌，可能包括人类和作物病原体。实验将通过对酿酒酵母进行紫外线诱变后的实验进化实验和/或选择，然后进行遗传分析(整体分离分析)或NGS，以揭示抗附着和抗性的机制。

This project will investigate natural anti-fungal inhibitors and assess synergistic/antagonistic interactions with other agents, extending to mode-of-action characterization and the impact of environment or media-type on interactions. This will include tests to find agents to which drug resistant fungal isolates may be hypersensitive, e.g., 'collateral sensitivity' in azole-resistant strains of C. albicans. In addition, the project will investigate fungal anti-attachment polymers as a novel, passive means for controlling problematic fungi, potentially encompassing both human- and crop-pathogens. Experiments will use experimental evolution experiments and/or selection after UV mutagenesis in S. cerevisiae, followed by genetic analysis (bulk segregant analysis) or NGS, to reveal mechanisms of anti-attachment and resistance.