UCLA NHLBI Shared Microarray Facilities

描述（由申请人提供）： 原位合成的寡核苷酸和cDNA点样微阵列提供了重要的工具，需要通过共享资源以高质量和一致的质量以及合理的价格向NHLBI社区普遍提供。拟议中的加州大学洛杉矶分校DNA微阵列设施内的NHLBI共享资源将是一个功能广泛的中心，提供一系列的微阵列试剂和服务。目前的UCLA微阵列核心提供从微阵列性能到数据管理和交付的全方位服务。在广泛的校园支持下，加州大学洛杉矶分校DNA微阵列设施一直在使用斑点cDNA和原位合成寡核苷酸阵列提供基于微阵列的基因表达分析。在本申请中，我们提出了这些服务的扩展：1）提供实验设计方面的帮助。我们将在加州大学洛杉矶分校提供一系列教育项目，总体目标是创建一个知识渊博的微阵列用户社区。2)提供微阵列服务，以满足NHLBI研究者基地的需求。目前的设施将扩大，以满足能力增加的需要。3)提供微阵列数据的数据管理和统计分析。由于大规模的数据处理和处理对生物学家来说是一项艰巨的任务，我们建议提供专门的专家数据管理和统计援助。

DESCRIPTION (provided by applicant): Both in situ synthesized oligonucleotide and cDNA spotted microarrays provide important tools, which need to be made generally available to the NHLBI community at high and consistent quality and at a reasonable price through the shared resource. The proposed NHLBI Shared Resource within the UCLA DNA Microarray Facility will be a broadly functioning center to provide an array of microarray reagents and services. The current UCLA Microarray core provides a full service from microarray performance to data management and delivery. With broad campus support the UCLA DNA Microarray Facility has been functioning to provide microarray based gene expression analysis using spotted cDNA and in situ synthesized oligo arrays. In this application we propose an extension of these services: 1) To provide assistance in experimental design. We will offer a series of educational programs at UCLA with the overall goal of creating a knowledgeable microarray user community. 2) To provide microarray services to meet demands of NHLBI investigator base. The current facility will be expanded to meet the needs for increased capacity. 3) To provide data management and statistical analysis of microarray data. As large scale data handling and processing can be a daunting task for biologists, we propose to provide dedicated expert data managing and statistical assistance.