Affordable large population genomic DNA collections

描述(申请人提供)：已知种族来源的大量人类基因组DNA的可用性仍然是流行病学和疾病基因研究的一个长期问题。在这笔赠款中，两种宝贵的资源被结合起来，以解决DNA的可用性问题。分子分级公司(MSI)开发了一种称为多重置换扩增(MDA)的技术，该技术能够对完整的人类基因组进行106倍的扩增，忠实地代表原始模板，具有出色的遗传位点表示和高保真的序列表示。这项技术将被用来放大一套广泛的已存档的人类基因组DNA样本，这些样本代表亚洲、非裔美国人和高加索人口，代表亚洲、非裔美国人和高加索人口，这些样本是斯克里普斯研究所的比特勒博士在NIDDK资助的一个项目中收集的。这些DNA样本将使用条形码控制的自动化过程进行放大和排列，并将提供给NIH资助的其他研究人员。在这个SBIR的第一阶段，我们将建立放大和排列过程的自动化和质量控制。

DESCRIPTION (provided by applicant): The availability of large human genomic DNA sets of known ethnic origin remains a persistent problem for epidemiology and disease gene research. In this grant two valuable resources are combined to solve DNA availability problems. Molecular Staging Inc (MSI) has developed a technology, termed Multiple Displacement Amplification (MDA), that is capable of producing 106 fold amplification of whole human genomes that faithfully represent the original templates, with excellent representation of genetic loci and high fidelity of sequence representation. This technology will be used to amplify an extensive set of archived human genomic DNA samples of defined ethnic origin, representing Asian, African American and Caucasian populations, previously collected by Dr. Beutler of Scripps Research Institute during a project funded by NIDDK. These DNA samples will be amplified and arrayed using bar code controlled automated processes and will be provided to other NIH funded researchers. In Phase I of this SBIR we will set up automation and quality control of amplification and arraying processes.