TECHNIQUES FOR MODELING HUMAN CANCER IN MICE

为了推进人类癌症的病因、治疗和预防的研究，迫切需要具有可遗传癌症的准确、可重现的人类癌症模型的小鼠模型。为了充分利用这些模式，调查必须是多学科的，利用不同领域的研究人员的专业知识。对小鼠生物学和病理学的理解是必不可少的，然而，使用其他模型系统或接受过其他研究学科培训的研究人员带来了同样重要的见解。为了实现以癌症控制和预防为重点的跨学科、转化性研究，必须为年轻的研究人员提供机会，让他们在准备和分析人类癌症的老鼠模型的技术方面进行动手培训。为了满足这一需求，杰克逊实验室(TJL)将就特定人类癌症的小鼠模型提供年度研讨会系列；第一个研讨会将重点放在乳腺癌的小鼠模型上。后续研讨会的主题将在咨询NCI小鼠模型人类癌症联盟指导委员会、TJL教员和其他癌症研究专家后确定。教师将从TJL的工作人员和特邀专家中挑选；课程将包括讲座和动手培训。参与者将处于博士后后期/早期独立研究人员水平，并将以竞争性方式选拔。第一个研讨会的具体目标是：1)演示繁殖和保持高健康水平的基因定义的突变菌株所需的基本畜牧业技术；2)演示在正常生殖周期和致癌过程中分析乳腺解剖的技术；3)演示在体内和体外操作乳腺生长的技术；4)介绍分析乳腺功能和肿瘤进展的新技术。

Mouse models with heritable cancers that are accurate, reproducible models of human cancers are urgently needed to advance research into the etiology, treatment, and prevention of human cancers. To exploit these models fully, investigations must be multi-disciplinary, drawing on the expertise of researchers in diverse fields. Understanding of mouse biology and pathology are essential, however, investigators using other model systems or who were trained in other research disciplines bring equally important insights. To achieve a cross-disciplinary, translational research focus on cancer control and prevention, opportunities must be provided for young investigators to have hands-on training in the techniques for preparing and analyzing mouse models of human cancers. To address this need The Jackson Laboratory (TJL) will offer an annual workshop series on mouse models of specific human cancers; the first will focus on mouse models for mammary cancer. The topics of subsequent workshops will be determined after consultation with the NCI Mouse Models of Human Cancers Consortium Steering Committee, TJL faculty, and other experts in cancer research. Faculty will be drawn from TJL staff and invited experts; sessions will include both lectures and hands-on training. Participants will be at the late-postdoctoral/early independent researcher level and will be competitively selected. The specific objectives of the first workshop are to: 1) demonstrate basic animal husbandry techniques needed to propagate and maintain genetically-defined mutant strains at high health levels; 2) demonstrate techniques for analyzing mammary gland anatomy throughout the normal reproductive cycle and during carcinogenesis; 3) demonstrate techniques for in vivo and in vitro manipulation of mammary gland growth; 4) introduce novel technologies for analyzing mammary gland function and neoplastic progression.