Mechanisms and Therapy of Ischemic Sudden Cardiac Arrest

增加由缺血和梗塞引起的心脏骤停的存活率，这是一个非常健康的问题，需要更好地理解引起心脏骤停的心律失常的机制和复苏尝试期间遇到的问题。该计划项目申请建议在动物中使用电学和光学映射技术来获得这些信息。项目1（猝死的缺血模型的长期监测）将从长期装备的动物遥测，并使用电标测来了解缺血和梗死如何、何时以及为什么发生猝死。项目2（缺血和梗死中VF维持的机制）将使用光学和电学标测来量化维持VF的机制。项目3（缺血和梗死中的浦肯野-心肌折返）将使用电标测来研究浦肯野纤维在引发心律失常中的作用。项目4（缺血性心律失常期间的除颤机制）将使用电标测来确定复苏期间缺血中除颤升高和再纤颤高发生率的机制。项目5（无脉性电活动的电治疗）将使用电和最佳标测来研究新的电治疗如何在复苏过程中消除无脉性电活动。核心A（行政）将管理并为项目提供生物统计和手稿准备支持。核心B（仪器和软件）将提供和软件支持。核心C（动物模型和病理学）将确保最佳的动物护理和病理学检查。

Increasing survival from sudden cardiac arrest caused by ischemia and infarction, an extremely health problem, requires better understanding of the mechanisms both of the arrhythmias causing it and of the problems encountered during resuscitation attempts. This program project application proposes to use electrical and optical and optical mapping techniques in animals to obtain this information. Project 1 (Chronic monitoring of ischemic models of sudden death) will telemeter from chronically instrumented animals and use of electrical mapping to understand how, when, and why sudden death occurs with ischemia and infarction. Project 2 (Mechanism of VF maintenance in ischemia and infarction) will use optical and electrical mapping to quantify the mechanisms maintaining VF. Project 3 (Purkinje-myocardial reentry in ischemia and infraction) will use electrical mapping to investigate the role of Purkinje fibers in initiating arrhythmias. Project 4 (Defibrillation mechanisms during ischemic arrhythmias) will use electrical mapping to determine the mechanisms for the elevated defibrillation and high incidence of refibrillation in ischemia during resuscitation. Project 5 (Electrical therapy for pulseless electrical activity) will use electrical and optimal mapping to study how new electrical therapies eradicate pulseless electrical activity during resuscitation. Core A (Administration) will administrate and provide biostatistical and manuscript preparation support for the project. Core B (Instrumentation and Software) will provide and software support. Core C (Animal models and pathology) will insure optimal animal care and pathological examination.