Purchase of 800 MHz NMR Spectrometer

一个由三所大学组成的联盟：位于博尔德的科罗拉多大学、科罗拉多大学健康科学中心（UCHSC）和犹他州大学医学院将购买一台800 MHZ NMR光谱仪，并得到NCRR共享仪器和NSF多用户生物仪器计划的部分支持。这场核磁共振将有利于七个主要用户的研究计划（A。Pardi和D.S. Wuttke at Boulder; W.L. Sundquist和D.R.戴维斯在犹他州;和B.K. UCHSC的Bendiak、D.N.M Jones和M. J. Overduin）和这三个机构的几个小用户。TROSY型实验的最新发展允许在比以前可能的大得多的大分子上获得极高分辨率的NMR光谱。这些TROSY实验在超高磁场下进行了优化，所需的800 MHz NMR将使主要用户能够利用这些强大的新技术。该仪器将被安置在基础科学大楼，这是新校园的一部分，目前正在建设的UCHSC在丹佛。主要用户目前可以使用500和600 MHz NMR仪器，因此所要求的800 MHz NMR将用于对800 MHz光谱仪的更高场、更高分辨率和更高灵敏度有明显需求的项目。所要求的仪器将加速以下领域的新的和正在进行的研究项目：-寡糖结构和细胞表面碳水化合物-蛋白质相互作用- B.K. Bendiak -HIV引物/模板复合物的结构研究-D.R.戴维斯-结构功能研究蛋白质和碳水化合物参与疾病- D.N.M.琼斯-结构，相互作用和膜相关蛋白质的动力学-M. J. Overduin -RNA-A的结构-功能研究。Pardi -HIV-1组装和出芽的结构研究- W.I. Sundquist -生物重要分子识别的结构研究- D.S. Wuttke

A consortium of three Universities: The University of Colorado, Boulder, the University of Colorado Health Science Center (UCHSC) and the University of Utah School of Medicine will purchase an 800 MHZ NMR spectrometer with partial support from the NCRR Shared Instrumentation and NSF Multi-User Biological Instrumentation Program. This field NMR will benefit the research programs of the seven major users (A. Pardi and D.S. Wuttke at Boulder; W.L. Sundquist and D.R. Davis at Utah; and B.K. Bendiak, D.N.M Jones, and M.J. Overduin at UCHSC) and several minor users at the three institutions. Recent developments in TROSY-type experiments allow acquisition of extremely high resolution NMR spectra on much larger macromolecules than was previously possible. These TROSY experiments are optimized at ultra-high magnetic fields, and the requested 800 MHZ NMR will make it possible for the major users to take advantage of these powerful new techniques. The instrument will be housed in the basic science building that is part of the new campus currently being constructed for UCHSC in Denver. The major users currently have access to 500 and 600 MHZ NMR instrumentation so the requested 800 NMR will be used for projects that have a demonstrated need for a higher field, higher resolution and higher sensitivity of the 800 MHz spectrometer. The requested instrument will accelerate new and ongoing research projects in the following areas: - Oligosaccharide Structures and Cell Surface Carbohydrate-Protein Interactions- B.K. Bendiak - Structural Studies of HIV Primer/Template Complex-D.R. Davis - Structural Function Studies of Proteins and Carbohydrates Involved in Disease- D.N.M. Jones - Structures, Interactions and Dynamics of Membrane-Associated Proteins- M.J. Overduin - Structure-Function Studies of RNA-A. Pardi - Structural Studies of HIV-1 Assembly and Budding- W.I. Sundquist - Structural Studies of Biologically Important Molecular Recognition- D.S. Wuttke