LC/quadrupole ion trap mass spectrometer

共价蛋白质结构的阐明在生物医学研究的各个领域都是一个有价值的工具。这一目标，即鉴定蛋白质序列和翻译后修饰，通过质谱学得到了独特的促进。在为分析生物样品而设计的质谱仪中，四重离子捕获器提供了卓越的灵敏度和高质量的裂解数据，以及与液相色谱的耦合。出于这些原因，我们建议从校园内获得一份LCCQ，DECA的调查人员将使用这一工具，其中包括一个由七名NIH RO1受赠者组成的主要用户组。该仪器将用于以下正在进行的资助研究：BM Willardson博士将在他对G蛋白调节蛋白的光导蛋白家族在调节信号转导中的作用的研究中确定磷酸化位点、相互作用的蛋白质和转录因子。MB Andrus博士将从他的实验室合成的溶液相天然产物样库中确定受体结合药物候选药物。TS Elton博士将确定在调节血管紧张素受体表达方面发挥作用的DNA和RNA结合蛋白。PB Savage博士将从他的实验室合成的阳离子类固醇三酰胺的组合文库中鉴定和表征与Lipid A结合的抗微生物候选药物。Dl Simmons博士将研究环氧合酶2和核结合蛋白的翻译后修饰，这是他研究这些蛋白质在癌症发生和细胞凋亡中作用的一部分。GF Burton博士将确定与滤泡树突状细胞在艾滋病发病机制中的功能有关的蛋白质。WW Winder博士将确定翻译后修饰、激酶靶标和转录因子，这是他正在进行的运动和2型糖尿病生理学研究的一部分。LC Q质谱仪使这些研究成为可能，否则这些研究是不可能实现的。

The elucidation of covalent protein structure is a valuable tool in every field of biomedical research. This goal, the identification of protein sequence and post-translational modifications, is uniquely facilitated through mass spectrometry. Among mass spectrometers designed to analyze biological samples, quadruple ion trap instruments offer superior sensitivity and high quality fragmentation data, as well as coupling to liquid chromatography. For these reasons, we propose to obtain a LCQ DECA investigators from across campus will make use of this instrument, including a Major Users Group consisting of seven NIH RO1 grantees. The instrument will be used in the following ongoing funded research: Dr. BM Willardson will identity phosphorylation sites, interacting proteins, and transcription factors in his investigations of the roles of the phosducin family of G-protein regulators in modulating signal transduction. Dr. MB Andrus will identify receptor-binding drug candidates from solution-phase natural product-like libraries synthesized in his lab. Dr. TS Elton will identify both DNA and RNA binding proteins that play roles in regulating the expression of the angiotensin receptor. Dr. PB Savage will identify and characterize Lipid A-binding anti-microbial candidates from combinatorial libraries of cationic steroid triamides synthesized in his lab. Dr. DL Simmons will investigate the post-translational modifications of cyclooxygenase 2 and nucleobindin, as part of his research into the roles of these proteins in carcinogenesis and apoptosis. Dr. GF Burton will identify proteins implicated in the function of follicular dendritic cells in AIDS pathogenesis. And Dr. WW Winder will identify post-translational modifications, kinase targets, and transcription factors as part of his ongoing research into the physiology of exercise and type 2 diabetes. The LCQ mass spectrometer enables these investigations which would not be feasible otherwise.