MDS Nordian Gammacell-40 Exactor Whole Animal Irradiator

为MDS Nordian公司申请资金。Gammacell-40精确的独立低剂量率研究动物（啮齿动物）辐照器，以支持正在进行的美国国立卫生研究院资助的个人发起的研究项目，该项目位于宾夕法尼亚大学医学院校园内最近建立的研究大楼（BRBII）内的一个新的最先进的动物设施。12个核心实验室包括所要求的仪器的主要用户清单。每个人都在BRBII有实验室，他们的老鼠将被安置在这里，并进行正在进行的实验，需要对整个动物进行辐射，并在这栋建筑的地下室对正在进行的实验中的动物进行长期的辐射维护。这些核心实验室将占辐照器使用量的大部分（约75%）。此外，还有25个以上的额外用户将很少（<25%）使用该工具。后一组由美国国立卫生研究院资助的宾夕法尼亚大学医学院相关部门、研究所和医院的教师以及在BRBII拥有实验室的其他教师组成。虽然校园内有另外两个类似的仪器，但由于核心用户组动物所在的新动物设施是一个封闭的设施，因此无法将带到其他建筑物进行辐照的动物送回调查员指定的区域，因此对这台额外仪器的需求特别大。因此，不可能进行需要在辐照后长期维持动物超过一天的实验，也不可能进行需要比较辐照和重建模型、骨髓嵌合体、免疫缺陷诱导等的实验。宾夕法尼亚大学医学院已作出重大财政承诺，支持采购这一仪器。耗资超过33,000美元，学校已经承诺为持续的服务合同提供资金来维护和维修仪器，承诺支付资金来补偿个人监督仪器的日常操作，并同意支付散热器的移动和安装费用。科学监督将由一个委员会完成，该委员会由提案的PI，亚伯拉罕森家庭癌症研究所的科学主任和核心用户组的两名教师代表组成。

Funds are requested for a MDS Nordian Inc. Gammacell-40 exactor self- contained low dose rate research animal (rodent) irradiator to support the ongoing NIH-funded individual-initiated research programs in a new state of the art animal facility located within a recently erected research building (BRBII) on the campus of the University of Pennsylvania School of Medicine. Twelve core laboratories comprise the list of the major users of the requested instrument. Each have laboratories in the BRBII and will house their mice and perform ongoing experiments requiring whole animal irradiation and extended maintenance of irradiated animals in ongoing experiments in the animals in the basement of this building. These core laboratories will constitute the majority (>75%) of the usage of the irradiator. In addition, there is a group of over 25 additional users that will utilize this instrument on an infrequent (<25% basis. This latter group is comprised of NIH-funded faculty from University of Pennsylvania School of Medicine associated departments, institutes, and hospitals in addition to other faculty with laboratories in the BRBII. Although two other similar instrument are available on the campus, a specific need for this additional instrument arises because the new animal facility in which the animals of the core user group are housed is a closed facility in which the animals taken to other buildings for irradiation cannot be returned to the investigator's designated area. As a consequence, it is impossible to perform experiments that are either long-term requiring maintaining the animals beyond a single day after irradiation, or require comparison of irradiated and reconstituting models, bone marrow chimeras, induction of immunodeficiency, among others. The University of Pennsylvania School of Medicine has already made a significant financial commitment to support the procurement of this instrument. At a cost of over $33,000, the school has committed funds for an ongoing service contract to maintain and service the instrument, committed funds to compensate an individual to oversee the day to day operation of the instrument, and agree to pay for the moving and setup costs for the irradiator. Scientific oversight will be accomplished by a committee comprised of the PI of the proposal, the scientific director of the Abrahamson Family Cancer Research Institute, and two faculty representatives from the core user group.