BIACORE 3000 Biosensor

描述(由申请人提供)：这是一份来自不同国家的申请 医学系、免疫学系、 药理学和癌症生物学公司将购买Biacore 3000生物传感器以支持 美国国立卫生研究院正在资助的10个免疫学、血液领域的研究项目 蛋白质和信号转导。杜克大学目前有三所 最先进的生物传感器，一台Biacore 2000和两台Biacore 3000仪器 它们仅在有限的基础上对提议的用户组可用。一 然而，旧的Biacore 1000生物传感器和一个IASys生物传感器是可用的， 这些仪器缺乏执行操作所需的灵敏度和吞吐量 调查组的申请将需要超过 占仪器总使用量的95%。访问该仪器将 根据内部咨询的建议向其他用户提供 委员会审议阶段。该仪器将被整合到 杜克大学人类免疫学核心设施和杜克大学专业研究中心 (SCOR)在类风湿关节炎中。继续为该行动提供财政支助 仪器的维护将由按存储容量使用计费机制提供 并得到了医学部的额外支持。一位技术高超的 杜克大学专门招募了一名调查员 医学部监督医院的日常运作 乐器。医学部还将为以下人员提供工资支持 将成为仪器专职操作员的技术人员。总而言之， 这组调查人员有明显的需求，并正在请求支持 用于最先进的Biacore 3000生物传感器。咨询委员会，机构 支持，对持续维护和管理计划的财务支持是 以确保该工具将充分和适当地 被利用了。

DESCRIPTION (provided by the applicant): This is a application from a diverse group of six investigators within the Departments of Medicine, Immunology, Pharmacology and Cancer Biology to purchase a BIAcore 3000 biosensor to support 10 ongoing NIH funded research projects in the areas of immunology, blood proteins and signal transduction. Duke University currently has three state-of-the-art biosensors, one BIAcore 2000 and two BIAcore 3000 instruments which are available to the proposed user group on only a limited basis. One older BIAcore 1000 biosensor and one IASys biosensor are available, however, these instruments lack the sensitivity and throughput required for performing the applications of the group of investigators which will require greater than 95 percent of the total usage of the instrument. Access to the instrument will be made available to other users based upon the advice of an internal advisory committee. The instrument will be integrated into the core facilities of the Duke Human Immunology Core Facility and the Duke Specialized Center of Research (SCOR) in Rheumatoid Arthritis. Continued financial support for the operation and maintenance of the instrument will be provided by a charge back mechanism with additional support from the Department of Medicine. A highly skilled investigator has been specifically recruited to Duke University by the Department of Medicine to supervise the day-to-day operations of the instrument. The Department of Medicine will also provide the salary support for a technician who will be the dedicated operator of the instrument. In summary, this group of investigators has a demonstrated need and is requesting support for state-of-the-art BIAcore 3000 biosensor. Advisory committees, institutional support, financial support for continued maintenance and management plans are in place to insure that the instrument will be fully and appropriately utilized.