Purchase of a Circular Dichroism Spectrometer

我们正在寻求资金来替换已有16年历史的Jasco J-500圆二色性光谱仪，这是目前佛罗里达大学唯一的圆二色性光谱仪。提议的仪器将是最先进的，将包括一个止流附件，一个自动滴定附件，荧光检测能力和一个控制样品温度的热电装置。来自佛罗里达大学三个学院和七个系的13位研究者（包括7个nih资助的项目）为这种非常重要和非常基础的仪器做出了研究证明。该仪器将成为一个新的结构生物学、生物物理仪器（SBBI）设施的一部分，并将补充现有的核磁共振、x射线晶体学、光学显微镜和计算设施。这些设施和7个教师研究小组构成了佛罗里达大学不断扩大的结构生物学项目的核心，拟议的CD光谱仪将成为美国国立卫生研究院资助的研究工作、其他联邦资助的研究工作和结构生物学研究生教育的病毒组成部分。CD光谱仪将设在佛罗里达大学麦克奈特脑研究所（MBI-UF），因为MBI-UF是佛罗里达大学结构生物学小组和仪器的焦点。作为机构支持的一部分，MBI-UF将为该仪器提供为期3年的空间、技术和计算机支持。佛罗里达大学将额外提供3年的维护费用和服务对比，使该仪器免费并随时可供所有佛罗里达大学的研究人员使用，优先事项如下：本提案的贡献者（75%），其他nih资助的项目，其他项目，研究生或本科教育。

We are seeking funds to replace 16 year old Jasco J-500 circular dichroism (CD) spectrometer, currently the only CD instrument at the University of Florida. The proposed instrument will be state-of-the-art and will include a stopped flow accessory, an automated titration accessory, fluorescence detection capabilities, and a thermoelectric device for controlling sample temperature. Thirteen investigators (including seven NIH-funded projects) from three colleges and seven departments in the University of Florida have contributed research justifications for this very important and very basic instrumentation. The proposed instrument will be part of a new structural biology, biophysical instrumentation (SBBI) facility and will complement existing NMR, x- ray crystallography, optical microscopy, and computational facilities. These facilities and seven faculty research groups form the core of an expanding structural biology program at the University of Florida, and the proposed CD spectrometer will be a viral component for NIH-funded research efforts, other federally funded research efforts, and graduate education in structural biology. The CD spectrometer will be located in the McKnight Brain Institute of the University of Florida (MBI-UF) because MBI-UF is the focal point for structural biology groups and instrument at UF. As part of the institutional support, MBI-UF will provide space, technical, and computer support for the instrument for 3 years., The University of Florida will additionally provide maintenance costs and service contrasts for 3 years, making the instrument free and readily available for all UF researchers, in the following priorities: contributors to this proposal (75%), other NIH-funded projects, other projects, and graduate or undergraduate education.