Core--Antibody

该抗体核心将产生单克隆抗体（mab），用于研究伽玛疱疹病毒感染的口腔发病机制。产生的单克隆抗体将用于(1)表征病毒抗原和(2)研究对已知病毒抗原的免疫反应。项目1将生成针对恒河淋巴隐病毒（LCV）基因产物的单克隆抗体，用于研究潜伏性和溶解性感染。此外，在该项目的后期，我们提出将急性恒河猴对LCV的反应与鼻咽癌（NPC）个体的抗体库联系起来。将生成针对RRV的单克隆抗体（项目2），用于研究卡波西肉瘤相关疱疹病毒（KSHV）蛋白，并将用于免疫组织化学研究KSHV基因在口腔中的表达（项目3）。人单克隆抗体，从感染KSHV的个体的B细胞中产生。人类单克隆抗体的开发和抗体反应的研究一直是实验室长期关注的焦点。我们与病毒学、微生物学和肿瘤学领域的其他机构有许多合作，研究抗体对自然感染/疾病、疫苗和癌症的反应。这一经验与有效核聚变伙伴的可用性相结合将有助于该核心的成功。

The antibody core will generate monoclonal antibodies (Mabs) for the study of oral pathogenesis of gammaherpesvirus infections. Monoclonal antibodies will be generated to be used to (1) characterize viral antigens and (2) to study the immune response to known viral antigens. For project 1, Mabs will be generated to rhesus lymphocryptovirus (LCV) gene products to study latent and lytic infection. Additionally, we proposed in later years of the project, to correlate the acute rhesus macaque response to LCV with the antibody repertoire of individuals with nasopharyngeal carcinoma (NPC). Mabs will be generated to RRV (project 2) to study Kaposi's sarcoma-associated herpesvirus (KSHV) proteins and will be used in immunohistochemistry to study KSHV gene expression in the oral cavity (Project 3). Human-Mabs, produced from the B cells of individuals infected with KSHV. The development of human monoclonal antibodies and the study of antibody responses have been a long-standing focus of the laboratory. We have numerous collaborations with others in the field of virology, microbiology and oncology to study antibody responses to natural infections/diseases vaccines and cancer. The combination of this experience and the availability of efficient fusion partners will contribute to the success of this core.