Oestrogenic Profiling in Lung in Pulmonary Arterial Hypertension by Mass Spectrometry Imaging

我的研究项目的主要重点是检查肺动脉高压（PAH）模型中的雌激素代谢，以便进行性别之间的比较研究，以及使用LC-MS/MS评估各种形式的疾病（遗传性，特发性）之间的异同。我还将使用MS成像探索雌激素及其代谢物在肺组织横截面（多环芳烃啮齿动物模型）中的区域表面分析，这将与雌激素的组织衍生化和化学标记的协议发展相结合。非靶向MS方法的进一步发展有望提供更广泛的空间脂质组学变化和肺部受多环芳烃影响的变化。这项研究的结果将用于发展我们对雌激素和相关代谢物在多环芳烃增殖中的作用的理解。然后，这些信息可用于确定潜在的诊断标记物，并强调治疗该疾病的新靶点。

The primary focus of my research project is the examination of oestrogen metabolism in a models of pulmonary arterial hypertension (PAH) to allow for a comparative study between the sexes, as well as an assessment of the similarities and differences between the various forms of the disease (genetic, idiopathic) using LC-MS/MS. I will also be exploring regional surface profiling of oestrogens and their metabolites in cross-sections of lung tissue (rodent models of PAH) using MS imaging, which will be coupled with the development of a protocol for the on-tissue derivatisation and chemical tagging of oestrogens. Further development of an untargeted MS approach will hopefully provide an insight into wider spatial lipidomic variations and changes in lungs affected by PAH. The results of this research will be used to develop our understanding of the role of oestrogens and associated metabolites in the proliferation of PAH. This information could then be used in the identification of potential diagnostic markers and highlighting new targets for treatment of the disease.