CORE--ANIMAL AND CELLULAR LABORATORY

描述(直接取自应用程序)：的总体目标 这个核心实验室 是为个别科学项目中的研究人员提供访问 到可靠且可重复性维护的整体动物和细胞模型 用于研究胰岛素分泌的基本分子机制及其机制 糖尿病的改变和程序性细胞死亡的调节 细胞内钙离子。这个核心实验室提供的服务将是 被所有三个科学项目所利用。具体目标是：1. -22-提供 调查人员获得专门定义的动物模型 胰岛β细胞功能障碍与自发性糖尿病 非糖尿病对照动物。这些模型包括点头鼠标，一种模型 自身免疫β细胞的破坏，糖尿病引起的NOD小鼠 通过免疫疗法和NOD SCID/SCID小鼠进行预防 糖尿病已通过使用糖尿病患者的脾细胞进行转移 点头老鼠。自发性糖尿病模型ZDF大鼠和db/db小鼠 减少的β细胞质量也将可用。2.隔离 从动物模型中制备分散的胰岛细胞 上面列出的以及正常大鼠和小鼠的β细胞功能障碍。3.至 在培养中保持包括BetaTC3，Min-6， HIT-T15和RINm5F等细胞株用于表达β细胞 特异性基因、相关基因亚型和稳定表达的细胞系 (例如，COS-7细胞)。4.进行灌流实验，研究 正常或糖尿病啮齿动物胰岛胰岛素分泌的调节 或者来自组织培养中保持的β细胞系。5.执行 研究β细胞胞内调控的灌流实验 组织培养中维持的胰岛和细胞系中的钙离子。

Description (taken directly from the application): The overall goal of this Core Laboratory is to provide investigators in the individual Scientific Projects access to reliable and reproducibly maintained whole animal and cellular models for the study of basic molecular mechanisms of insulin secretion and its alterations in diabetes and the regulation of programmed cell death by intracellular Ca2+. The services provided by this Core Laboratory will be utilized by all three Scientific Projects. The Specific Aims are: 1. To - 22 -provide investigators access to specially defined animal models of beta-cell dysfunction and spontaneous diabetes and the appropriate non-diabetic control animals. These models include the NOD mouse, a model of autoimmune beta-cell destruction, the NOD mouse in which diabetes has been prevented by immunotherapy and the NOD scid/scid mouse in which diabetes has been adoptively transferred using spleen cells from diabetic NOD mice. The ZDF rat and the db/db mouse, models of spontaneous diabetes and reduced beta-cell mass will also be made available. 2. To isolate islets and prepare dispersed islet cells from the animal models of beta-cell dysfunction listed above and from normal rats and mice. 3. To maintain in culture, beta-cell tumor lines including betaTC3, MIN-6, HIT-T15 and RINm5F cells and other cell lines used to express beta-cell specific genes, related gene isoforms and stably transfected cell lines (e.g., COS-7 cells). 4. To perform perifusion experiments to study the regulation of insulin secretion in islets from normal or diabetic rodents or from beta-cell lines maintained in tissue culture. 5. To perform perifusion experiments to study the regulation of beta-cell intracellular Ca2+ in islets and cell lines maintained in tissue culture.