Core A: Islet Cell Biology Core

核心 A：胰岛细胞生物学核心

• 批准号: 8626377
• 负责人: Louis H. Philipson
• 金额: $ 18.78万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-01 至 2018-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8626377
• 关键词: Adenovirus Vector; Adenoviruses; Anabolism; Animal Model; Apoptosis; Beta Cell; Biochemical; Biology; Calcium; Cell Line; Cell physiology; Cells; Cellular biology; Data; Diabetes Mellitus; Diabetic mouse; Disease; Electrophysiology (science); Endocrine; Ensure; Foundations; Functional disorder; Funding; Genes; Goals; Health; Human; Image; In Vitro; Instruction; Insulin; Ion Channel; Islet Cell; Islets of Langerhans; Libraries; Medical Students; Methods; Movement; Mus; Phosphorylation; Physiological; Procedures; Research; Research Design; Research Personnel; Research Training; Secretory Vesicles; Services; Spectrum Analysis; Students; Techniques; Technology; Total Internal Reflection Fluorescent; Training; Underrepresented Minority; Woman; base; biophysical techniques; cell type; diabetic; high school; in vivo; insulinoma; islet; novel; protein function; repository; research study; tool; undergraduate student

The Islet Cell Biology Core provides services and hands-on training to independently funded investigators in the isolation and functional characterization of pancreatic islets from normal and diabetic humans and mice. It also maintains a repository of insulinoma cell lines as well as other endocrine cell lines. The primary emphasis of the Core is to facilitate studies of primary islet cells, and it has developed many unique tools and techniques for carrying such studies including novel animals models, biophysical methods and a library of adenovirus-based expression constructs for studying beta-cell function. The long-range objectives and goals of the Islet Cell Biology Core are to provide state-of-the-art technology and know-how to understanding the beta cell in health and disease. It has the following Specific Aims and Objectives: Provide advice, service and training in the isolation of pancreatic islets from normal and diabetic mice and humans; Provide insulinoma and other endocrine cell lines; Provide advice, service and training in the characterization of mouse and human pancreatic islets and beta cell (insulin biosynthesis and secretion; biophysical methods for studying islet and beta-cell function (e.g. calcium imaging, electrophysiology, total internal reflection fluorescent microscopy); biochemical methods for studying beta-cell function (e.g. phosphorylation, proliferation, apoptosis); and immunohistochemical analysis of pancreatic islets) and Provide advice, service and training on the use of adenovirus-based expression constructs to study protein function in beta cells and other cell types in vitro and in vivo. Drs. Philipson, Rhodes and Witkowski are Directors of this Core. They will be advised by experts in pancreatic islets and beta cells (Prince and Steiner), cellular and physiological imaging (Bindokas, Chen and Glick), ion channels (Hanck and Nelson), spectroscopy (Halpern and Scherer) and cell dynamics (Dinner) to ensure that the Core services are at the forefront of technology and thus able to anticipate and quickly repond to users needs.

胰岛细胞生物学核心为独立资助的研究人员提供正常和糖尿病人类和小鼠胰岛的分离和功能表征方面的服务和实践培训。它还维护胰岛素瘤细胞系以及其他内分泌细胞系的储存库。核心的主要重点是促进原代胰岛细胞的研究，它开发了许多独特的工具和技术来进行此类研究，包括新颖的动物模型、生物物理方法和用于研究β细胞功能的基于腺病毒的表达构建体库。胰岛细胞生物学核心的长期目标是提供最先进的技术和专业知识来了解健康和疾病中的 β 细胞。它有以下具体目的和目标： 提供从正常和糖尿病小鼠和人类中分离胰岛的建议、服务和培训；提供胰岛素瘤等内分泌细胞系；提供小鼠和人类胰岛和β细胞表征的建议、服务和培训（胰岛素生物合成和分泌；研究胰岛和β细胞功能的生物物理方法（例如钙成像、电生理学、全内反射荧光显微镜）；研究β细胞功能的生物化学方法（例如磷酸化、增殖、 细胞凋亡）；和胰岛的免疫组织化学分析）并提供有关使用基于腺病毒的表达构建体在体外和体内研究β细胞和其他细胞类型的蛋白质功能的建议、服务和培训。博士。 Philipson、Rhodes 和 Witkowski 是该核心的董事。他们将得到胰岛和β细胞（Prince和Steiner）、细胞和生理成像（Bindokas、Chen和Glick）、离子通道（Hanck和Nelson）、光谱学（Halpern和Scherer）和细胞动力学（Dinner）专家的建议，以确保核心服务处于技术前沿，从而能够预测并快速响应用户需求。