Mechanisms of renal tubular epithelial cell injury

总体描述（由申请人提供） 急性肾衰竭的总死亡率保持在 大约50%。这种高死亡率和相关成本不太可能 将减少，直到我们有一个更好的理解细胞， 细胞损伤和恢复的分子机制。本计划项目补助金 应用程序代表了基础之间的多学科协作努力 科学家和临床科学家围绕一个中心主题， 肾小管上皮细胞损伤的细胞和分子基础， 包括从细胞凋亡研究中学到的概念。 参加这些项目的有六个部门的成员（医学， 病理学、微生物学和免疫学、老年病学、生物化学和生理学） 包括几位细胞生物学家，他们具有体外和体内细胞生物学方面的专业知识， 急性肾衰竭模型（Shah、Portilla、步行者、Safirstein）， 具有分子生物学专业知识的研究人员（Crew，Portilla，Haun， Price）、临床科学家（肾脏病理学家）（步行者），以及 在蛋白质纯化方面具有特殊专长的生物化学家（Kaushal， Basnakian）。其他优势包括：1.一些最初的观察 从细胞凋亡的研究中学到的概念与肾脏疾病的相关性， 在本申请中，参与者报告了肾小管损伤。2.的 项目主任、项目负责人以及其他合作研究人员都有一个 专业协会和出版合作研究的历史。的 所有项目负责人的实验室和办公室都位于 允许参与者之间的随时访问和交互。 3.参与该项目的几名研究人员以及 该机构药理学部门的其他研究者， 生理学和肾脏科正在进行的项目， 直接关系到这个赠款申请的中心主题，进一步 强化环境以实现应用程序的目标。程序 已经获得了大量的研究人员来解决发病机制， 急性肾衰竭的多方面，协同的方式，从而匹配 计划项目指导方针的总体既定目标。

OVERALL DESCRIPTION (Provided by the applicant) The overall mortality following acute renal failure has remained at approximately 50%. It is unlikely that this high mortality and associated cost will be reduced until we have a better understanding of the cellular and molecular mechanisms of cell injury and recovery. This program project grant application represents a multidisciplinary collaborative effort among basic scientists and clinician scientists around a central theme of elucidating the cellular and molecular basis of rental tubular epithelial cell injury, including concepts that have been learned from the study of apoptosis. Participating in the projects are members of six departments (Medicine, Pathology, Microbiology & Immunology, Geriatrics, Biochemistry and Physiology) and include several cell biologists with expertise with in vitro and in vivo models of acute renal failure (Shah, Portilla, Walker, Safirstein), investigators with expertise in molecular biology (Crew, Portilla, Haun, Price), clinician scientist who is a renal pathologist (Walker), and biochemists with particular expertise in protein purification (Kaushal, Basnakian). Additional strengths include: 1. Some of the original observations of the relevance of the concepts learned from the study of apoptosis to renal tubular injury were reported by participants in this application. 2. The program director, project leaders as well as other co-investigators have a history of professional association and publishing collaborative studies. The laboratories and offices of all project leaders are located in close proximity to each other allowing ready access and interaction between the participants. 3. Several of the investigators participating in the program project as well as other investigators at the institution in the Departments of Pharmacology, Physiology and the Division of Nephrology have ongoing projects that are directly related to the central theme of this grant application which further strengthens the environment to pursue the goals of the application. The program has attained a critical mass of investigators to address the pathogenesis of acute renal failure in a multifaceted, synergistic fashion thus matching the overall stated objectives of the program project guidelines.