CORE--PROTEIN EXPRESSION FACILITIES

产品描述： 杆状病毒表达系统将用于表达重组 该真核表达系统包括 许多翻译后修饰途径，使生产 重组蛋白的抗原性，免疫原性， 在功能上类似于天然蛋白质（例如MLCK、MAPK）。还 这种过量产生的蛋白质的大部分在昆虫细胞中保持可溶性， 病毒基因组相对较大（130 kbp）， 外源DNA片段（如肌球蛋白重链、CaD等）。的 双启动子共表达载体（例如p2Bac）允许同时 两种重组蛋白的表达（例如，肌球蛋白重链和轻链 链）。该核心将服务于项目1、2-A和2-C。

Description: The Baculovirus Expression System will be used for expression of recombinant proteins in eukaryotic cells This eukaryotic expression system encompasses many of the post-translational modification pathways, allowing the production of recombinant protein which is antigenically, immunogenically, and functionally similar to the native protein (e.g. MLCK, MAPK). Also, the majority of this overproduced protein remains soluble in the insect cells and the viral genome is relatively large (130 kbp) and thus can accommodate large segments of foreign DNA ( e.g. myosin heavy chain, CaD, etc.). The dual-promoter co-expression vector (e.g. p2Bac) permits simultaneous expression of two recombinant proteins (e.g., myosin heavy chain and light chains). This core will serve Projects 1, 2-A and 2-C.