Learning & memory dependent encoding in the hippocampus

描述（由申请人提供）：记忆对身份和 海马体是正常记忆所必需的。的因果 海马细胞活性与学习记忆的关系 然而，性能仍然未知，因为很少有实验记录神经元 当内存需求变化且行为的其他方面保持不变时触发 常数目标1.为了识别神经元编码中的学习依赖性变化， 记录海马神经元组，而大鼠执行的任务， 海马体需要海马体的成功的新学习应该 需要海马形成新的和持续的放电模式 神经元大鼠将在一个+迷宫任务中接受训练， 依赖或独立的策略。海马细胞群 将在行为相同但学习能力不同的大鼠中记录 能力、记忆策略和海马功能。探测器将测试 每只大鼠使用的策略，以及正常位置的单位活动将进行比较 和反应学习者，以及穹窿或尾状核损伤的大鼠。目标2.测试 目标1中结果的一般性，神经元活动将记录在 老鼠接受了一种独特的学习方式，即食物的社会传播。 偏好所有的神经元可能都需要一个动态神经元反应的子集。 依赖于校园的学习类型，独立于行为表达， 学习动机的类型和具体的学习内容。海马神经元 正常的大鼠应该发展持久的嗅觉之间的联系， 任意选择的新型食品气味和二硫化碳气味， 食品安全的信号。目标3。为了测试尖峰之间的时间关系， 训练编码记忆通过比较实时整体活动在 海马体。重要的存储器信息可以通过时间编码来编码。 神经元动作电位之间的关系。尖峰脉冲的时间动力学 在相同行为但不同记忆负荷期间记录的合奏中 将使用已公布的新方法进行评估。分析将开始， 当大鼠在右迷宫中穿过A +迷宫的起始臂时， 然后左转

DESCRIPTION (provided by applicant): Memory is crucial to identity and survival, and the hippocampus is required for normal memory. The causal relationship between hippocampal cell activity and learning and memory performance, however, remains unknown because few experiments record neuronal firing while memory demands are varied and other aspects of behavior are held constant. Aim 1. To identify learning-dependent changes in neuronal encoding by recording groups of hippocampal neurons while rats perform a task that requires the hippocampus. Successful newlearning that requires the hippocampus should require the formation of new and persistent firing patterns by hippocampal neurons. Rats will be trained in a + maze task that can be solved by either hippocampus-dependent or independent strategies. Groups of hippocampal cells will be recorded in rats that behave identically but differ in learning ability, memory strategy, and hippocampal function. Probes will test the strategies used by each rat, and unit activity will be compared in normal place and response learners, and rats with fornix or caudate lesions. Aim 2. To test the generality of the results in Aim 1, neuronal activity will be recorded in rats trained in a distinct type of learning, the social transmission of food preference. A subset of dynamic neuronal responses may be required for all types of hippocampus-dependent learning, independent of behavioral expression, type of motivation, and specific content of learning. Hippocampal neurons in normal rats should develop persistent olfactory associations between the arbitrarily selected novel food odors and the odor of carbon disulfide, the signal of food safety. Aim 3. To test if temporal relationships among spike trains encode memory by comparing real-time ensemble activity in the hippocampus. Significant memory information may be encoded by the temporal relationships among neurons action potentials. The temporal dynamics of spiking within ensembles recorded during identical behaviors but different memoryloads will be assessed using published and a new method. The analysis will begin by comparing spike records as rats traverse the start arm of a + maze during right and left turns.