BEHAVIORAL AND PHYSIOLOGIC PATHOBIOLOGY OF MICE

描述（由申请人提供）：主要研究者（PI）的长期职业目标是：1）继续以有意义的方式为睡眠机制和功能的基础研究做出贡献; 2）增加资源和培训，以支持SIU医学院生物医学研究的持续发展; 3）提倡人道和明智地使用动物模型的价值和必要性，以加速阐明人类疾病的原因，治疗，治愈和预防。 PI过去和现在的服务和行政职责限制了将新的研究方法引入实验室的时间，指导学生和其他学员，以及作为小鼠病理学专家积极参与合作研究。 该奖项提供的机构服务的救济将使PI有时间实现以下具体目标：1）发展新的分析技能，（微阵列技术）和行为[睡眠剥夺（SD），抑郁症和焦虑]的方法，用于在划定睡眠的免疫调节作用的兴趣应用; 2）增加指导工作，直接对学生和教师;以及3）发起合作，以促进和支持人类疾病小鼠模型的研究。 这项提案的研究目标集中在定义睡眠不足对健康的影响。嗜睡和睡眠质量差广泛影响一般健康状况的测量，特别是影响对能量，疲劳和自给自足的看法。 睡眠片段，非恢复性睡眠和睡眠不足通常被认为会对宿主的防御能力产生不利影响。然而，这些关系并没有被经验定义。 一些对动物的研究表明，睡眠不足或质量差可能会增加对疾病的易感性，加剧疾病症状或延迟康复。 本申请的主要研究目标是开发和使用适当的动物模型来评估睡眠、睡眠丧失与感染性或炎症性疾病的易感性或恢复之间的关系。

DESCRIPTION (provided by applicant): The Principal Investigator's (PI) long-term career objectives are to: 1) continue to contribute in a meaningful way to basic research on the mechanisms and functions of sleep; 2) enhance resources and training that will support the continued growth of biomedical research at the SIU School of Medicine; and 3) advocate the value of and need for the humane and judicious use of animal models to expedite the elucidation of causes, treatments, cures, and prevention of human disease. The PI's past and current service and administrative responsibilities has limited the time available for introducing new research methodology into the laboratory, for mentoring students and other trainees, and for actively participating in collaborative research as a specialist in mouse pathobiology. The relief from institutional service offered by this award will allow the PI the time to accomplish the following Specific Aims: 1) to develop skills in new analytic (microarray technology) and behavioral [sleep deprivation (SD), depression, and anxiety] methodologies for application to interests in delineating the immune modulatory role of sleep; 2) to increase mentoring efforts directed toward both students and faculty; and 3) to initiate collaborations that will promote and support the study of mouse models of human disease. Research goals for this proposal focus on defining the health implications of inadequate sleep. Sleepiness and poor sleep quality broadly influence measures of general health status, particularly impacting perceptions about energy, fatigue, and self-sufficiency. Sleep fragmentation, non-restorative sleep, and inadequate sleep are commonly assumed to adversely impact host defense capabilities. However, these relationships have not been empirically defined. Some studies in animals suggest that inadequate or poor-quality sleep may increase susceptibility to disease, exacerbate symptoms of disease, or delay recuperation. The development and use of an appropriate animal model to evaluate the relationships between sleep, sleep loss, and susceptibility to or recuperation from infectious or inflammatory disease is the primary research goal of this application.