Core--Molecular neurobiology

核心B旨在为中枢神经系统和外周的神经解剖学、神经化学和神经递质受体以及原位杂交研究提供集中资源。为分子药理学、总RNA的提取、溶液杂交-核酸酶保护试验、聚合酶链式反应和狭缝印迹RNA分析、蛋白质印迹分析以及TK多肽、BK和细胞因子的测定提供集中的资源。核心主任将负责监督核心的运作；合作调查员和顾问将负责培训调查员、研究助理和研究技术人员。通过提供中央设施，核心将促进解剖学、神经化学和分子药理学方法的标准化，并确保数据分析的统一标准。作为一项额外服务，核心将就使用药理工具操纵生理系统向调查人员提供咨询意见，并提供生物活性化合物中央资料库(药物资料库)，供个别调查人员使用。这项服务将消除生物活性物质的重复订购，并确保适当的受体药理方法，或购买必要的设备和用品。

Core B is designed to provide a centralized resource for neuroanatomical, neurochemical, neurotransmitter receptors in the CNS and in the periphery, and for in situ hybridization studies. To provide a centralized resource for molecular pharmacology, isolation of the total RNA, solution hybridization-nuclease protection assays, PCR assays and slot blot RNA analysis, Western blot analysis, and assays for measurements of Tk peptides, BK and cytokines. The core director will be responsible for overseeing operation of the core; co-investigators and consultants will be available to train investigators, research associates and research technicians. By providing a central facility, the core will facilitate standardization of anatomical, neurochemical and molecular pharmacological methods and assure uniform criteria for data analysis. As an additional service, the core will advise investigators concerning the use of pharmacological tools to manipulate physiological systems and provide a central repository of bioactive compounds (drug library) for the use of individual investigators. This service will eliminate duplicate ordering of bioactive agents and assure proper receptor pharmacological methods, or purchase of necessary equipments and supplies.