CORE--CELL IMAGING FACILITY

描述：此中心拨款申请再次申请资金，以支持 细胞成像核心。核心人员将包括吉布斯博士和朱迪思女士。 巴尔西塔和核心将负责所有相关的例行活动 以形态学为核心，包括组织准备和切片，股票 溶液制备、记录保存、探针制备、免疫组织化学 染色、原位杂交及其制备和发展 放射自显影。核心总监将投入20%的精力来管理核心， 并将担任设计和执行实验的顾问 涉及定量组织化学技术的使用，以及帮助 对成像设备的使用人员进行培训。他还将提供两个 每年举办一次讲习班，其中一次将侧重于电子图像在 准备演示文稿和出版物，另一个讲习班将 关注组织化学和细胞成像方法的进展。 尽管巴尔西塔女士将100%的努力投入到核心活动中，但只有 要求她工资的10%用于支持日常核心维护活动。 支持她工资的其余资金将从退款中获得 系统作为为中心补助金制作的工作的一项功能。

Description: This Center Grant application again requests funding to support a Cell Imaging Core. Core personnel will consist of Dr. Gibbs and Ms. Judith Balcita and the Core will be responsible for all routine activities associated with a Morphology Core, including tissue preparation and sectioning, stock solution preparation, record keeping, probe preparation, immunohistochemical staining, in situ hybridization, and the preparation and developing of autoradiograms. The Core Director will devote 20% effort to managing the Core, and will serve as a consultant on the design and execution of experiments involving the use of quantitative histochemical techniques, as well as help train personnel in the use of the imaging equipment. He will also provide two workshops a year, one of which will focus on the use of electronic images in the preparation of presentations and publication, and the other workshop will focus on advances in histochemical and cell imaging methods. Although Ms. Balcita will devote 100% of her efforts to Core activities, only 10% of her salary is requested to support routine Core maintenance activities. The rest of the funds to support her salary will be derived from a charge-back system as a function of work produced for the Center Grant.