CORE--HIGH RESOLUTION MICROSCOPY

描述：(申请人的描述)分子的探测和成像 在生物系统中是生物医学中日益重要的工具 研究。共焦显微镜已经成为这方面的领先技术 目的。 1998年，Kent Keyser博士获得了NIH NCRR共享仪器 GRANT，UAB健康服务基金会奖，以及来自UAB的启动资金 教务长办公室，医务处和 UAB的学术部门和中心主任。这些资金被用来 建立一个新的设施，高分辨率成像设施。这一成像 资源配备两台徕卡共焦成像分光光度计TCS SP 单位。其中一个被配置为使用相干Mira Ti进行多光子激发： SA超快激光器，输出可在690-990纳米之间选择 允许以最小的光毒性对活细胞和组织进行成像，以及 结构距离组织表面200-400微米。SP扫描头 为每个检测器通道提供单独的分光光度计，允许 选择发射光谱的特定部分以交付给 探测器。其结果是更高的荧光产量，减少串扰，以及 改善了图像质量。该设施还包括奥林巴斯FluoView CLSM 使用光纤发射的氪离子和氩离子激光器，传统的 荧光和DIC显微镜设备以及配备有 数字反卷积软件、输出和图像存储设备。在……里面 此外，通过与伯明翰老将S达成合作协议 行政医疗中心，癌症中心的成员将有权访问 徕卡TCS SP单元配备了紫外光激光器和紫外光校正光学元件。三个都是 徕卡仪器配备三个或四个探测器通道， 透射光通道，以及Ar离子、氪离子和He：Ne激光器。 这些系统能够同时成像多达三个荧光团，并且 也能够获得DIC图像。这些软件包提供了 三维重建，时间推移成像，比率成像， 生理学套装和形态测量程序。该基金将提供 综合癌症中心成员获得最先进的成像技术 配备齐全的仪器和使用这些设备的专业知识 富有成效的资源。

Description: (Applicant's Description) The detection and imaging of molecules in biological systems is an increasingly important tool in biomedical research. Confocal microscopy has become a leading technology for this purpose. In 1998, Dr. Kent Keyser was the recipient of an NIH NCRR Shared Instrument Grant, a UAB Health Services Foundation award, and start-up funds from the UAB Office of the Provost, the Chairs of Departments in both the Medical and Academic Divisions of UAB, and Center Directors. These funds were used to establish a new facility, the High Resolution Imaging Facility. This imaging resource is equipped with two Leica Confocal Imaging Spectrophotometer TCS SP units. One is configured for multiphoton excitation with a Coherent MIRA Ti: Sa ultrafast laser with output selectable between 690-990 nanometers This permits imaging of living cells and tissues with minimal phototoxicity, and of structures 200-400 micrometers from the tissue surface. The SP scan head provides an individual spectrophotometer for each detector channel, allowing selection of specific portions of the emission spectrum for delivery to the detector. The result is higher fluorescence yield, reduced crosstalk, and improved image quality. The Facility also includes an Olympus Fluoview CLSM with fiber launched Krypton ion and Argon ion lasers, conventional fluorescence and DIC microscopy equipment, and work stations equipped with digital deconvolution software, output and image storage devices. In addition, through a cooperative agreement with the Birmingham Veteran?s Administration Medical Center, Cancer Center members will have access to a Leica TCS SP unit equipped with a UV laser and UV corrected optics. All three Leica instruments are equipped with either three or four detector channels, a transmitted light channel, and Argon ion, Krypton ion and Helium: Neon lasers. These systems are able to image up to three fluorophores simultaneously and are capable of obtaining DIC images as well. The software packages offer three-dimensional reconstruction, time-lapse imaging, ratio imaging, physiology packages, and morphometry routines. The Facility will offer Comprehensive Cancer Center members access to a state-of-the-art imaging facility with a full range of instrumentation and the expertise to use these resources productively.