Understanding the effects of Natural Variation and Disease Stress on Meiotic Recombination in Arabidopsis thaliana

欧亚和非洲拟南芥资源间减数分裂重组数量性状位点（qRTL）的定位将利用简单序列长度多态性（SSLPs）对来自杂交的不同F2个体进行基因型分析。这些杂交包含减数分裂交叉报告基因，这意味着重组表型可以与SSLP基因型数据进行比较，以便对影响重组的qtl进行扫描。随着项目的进展，项目的方向可能会发展到更密切地关注着中心点区域，这些区域显示出高水平的结构多态性。此外，疾病和免疫信号对减数分裂重组的影响将被研究。拟南芥植株将同时感染天然和通用病原体，下一代将使用荧光或分子标记分析重组频率的任何变化。

PhD project strategic theme: Bioscience for sustainable agriculture and food Mapping meiotic recombination quantitative trait loci (qRTL) between Eurasian and African Arabidopsis thaliana accessions will utilise simple sequence length polymorphisms (SSLPs) to genotype different F2 individuals derived from the crossed. These crosses contain meiotic crossover reporters, meaning recombination phenotype can be compared to SSLP genotype data in order to perform a scan for QTLs influencing recombination. The direction of the project may evolve to look more closely at the centromeric regions as the project progresses, which show high levels of structural polymorphisms between accessions. Furthermore, the effect of disease and immunity signalling on meiotic recombination will be investigated. Arabidopsis plants will be infected with both natural and generalist pathogens and the next generation will be analysed for any changes in recombination frequency using fluorescent or molecular markers.