Mechanisms of Nucleic acid Uptake by Renal Epithelium

描述(由申请人提供)： 对疾病过程进行干预的机会之一是确定 参与并随后抑制它们的分子。从理论上讲，这可以通过 识别编码蛋白质的核酸并使用互补的 阻止这种情况发生的DNA链。不幸的是，虽然这个概念 在流体的溶液中工作，在细胞中做到这一点的能力 很难。其中一个主要问题是了解这些链是如何 的DNA进入细胞。本项目的目的就是探讨这一问题。我们 之前已经证明了单链DNA可以通过高度 特定频道。我们纯化了这个蛋白质复合体，并一直在鉴定 这个综合体的每个组成部分都有什么作用。我们现在已经确定了其中一个 这一通道的亚基作为一种常见的酶，也起到调节DNA的作用 领悟。目前的提案将评估其他次级单位和参与方 这项活动。我们希望利用这些信息将DNA分子设计成 用于治疗疾病的治疗学。

DESCRIPTION (provided by applicant): One of the opportunities to intervene in a disease process is to identify the molecules that participate and then inhibit them. This can be done in theory by identifying the nucleic acids that encode the protein and using a complementary strand of DNA to block this from happening. Unfortunately, while the concept works in solutions of fluid, the ability to do this in a cell has been more difficult. One of the major problems has been understanding how these strands of DNA get into cells. The purpose of this project is to explore this issue. We have previously shown that single stranded DNA can move through a highly specific channel. We purified this protein complex and have been identifying what each component of this complex does. We have now identified one of the subunits of this channel as a common enzyme that serves also to regulate DNA uptake. The current proposal will evaluate other subunits and participants in this activity. We hope to use this information to design DNA molecules as therapeutics to treat disease.