MULTIPHOTON BIOMEDICAL NANOFABRICATION

描述（改编自申请人的摘要）：本申请寻求 开发一种新的方法来指导生物分子和合成物的三维组装， 聚合物这将通过在一个高度受控的环境中交联分子来完成。 使用多光子照明的方式。各种各样的生物分子 在保持其生物活性的同时并入3D结构中。的 研究人员声称，他们的方法比现有的方法更有优势， 包括光刻、微接触印刷和自由成形 微细加工这将通过更高的光学分辨率来实现， 保留生物功能，并提高三维能力。可行性 已经通过用合成聚合物制造结构来证明， 蛋白质、水凝胶和活性酶。拟议的工作将发展 本发明提供了在许多生物和医学领域具有广泛适用性的制造方法， 生物工程领域。具体目标是：1）制造结构， 在生物传感器、基因芯片、基于蛋白质的微型机器、 微米级的酶工厂和生物材料。2)为了制造纳米和 用于生物传感器、药物递送和组织的微尺度凝胶 工程. 3)为了制造尺寸复杂的通道和结构， 微流体的应用。4)制造复合材料和生物材料， 组织工程学

DESCRIPTION (adapted from applicant's abstract): This application seeks to develop a new method to direct the 3-D assembly of biomolecules and synthetic polymers. This will be done by cross-linking molecules in a highly controlled manner using mutiphoton illumination. A wide range of biological molecules will be incorporated in the 3-D structures while maintaining their bioactivity. The investigators claim that their approach provides benefits over current methods including photolithography, microcontact printing and free-form microfabrication. This will be accomplished with higher optical resolution, preservation of biological function, and improved 3-D capability. Feasibility has been demonstrated by fabricating structures with synthetic polymers, proteins, hydrogels, and active enzymes. The proposed work will develop fabrication methods with wide applicability in a number of biological and bioengineering fields. Specific goals are: 1) to fabricate structure for applications in biosensors, gene chips, protein-based micro-machines, micrometer scale-enzyme factories, and biomaterials. 2) To fabricate nano and micro-scale gels for application in biosensors, drug delivery, and tissue engineering. 3) To fabricate dimensionally complex channels and structures for applications to microfluidics. 4) To fabricate composites and biomaterials for tissue engineering.