Characterization of the roles of PP1 phosphatases in cytokinesis

博士项目战略主题：了解生命的规则细胞分裂忠实地将基因组信息分配在两个子细胞之间，这一过程中的错误与许多人类疾病有关。大多数有丝分裂事件是由磷酸化调节的，尽管有丝分裂激酶已经被研究得相当详细，但对它们的反作用磷酸酶知之甚少。宿主实验室最近表征了有丝分裂最后阶段（胞质分裂）中的蛋白质相互作用网络，并发现PP1磷酸酶最丰富和最常见。该项目的目的是使用结合基因编辑，靶向蛋白水解，定量蛋白质组学和多维高分辨率显微镜的多系统方法来表征这些磷酸酶在胞质分裂过程中的作用。

PhD project strategic theme: Understanding the rules of life Cell division faithfully partitions the genomic information between the two daughter cells and errors in this process have been implicated in many human diseases. Most mitotic events are regulated by phosphorylation and, although mitotic kinases have been studied is some detail, very little is known about their counteracting phosphatases. The host laboratory has recently characterised the protein interaction network in the last phase of mitosis, cytokinesis, and found that PP1 phosphatases were the most abundant and frequent. The aim of this project is to characterize the roles of these phosphatases during cytokinesis using a multi-systems approach combining gene editing, targeted proteolysis, quantitative proteomics, and multi-dimensional high resolution microscopy.