Targeted antigens for novel strategies of vaccination

描述（由申请人提供）：免疫过程包括抗原摄取和专业抗原呈递细胞（APCs）的加工，APCs的激活以及抗原承载细胞向淋巴器官的迁移。这反过来又会导致T淋巴细胞的激活。这一过程依赖于通常由微生物模式识别提供的炎症信号的存在。先前的研究表明，抗体分子为抗原呈递和疫苗接种提供了理想的递送载体。抗体可以有效地将相关抗原引导到特定的细胞亚群，如树突状细胞，同时满足细胞活化的要求。根据目标树突亚群的类型，预测会产生Th1或th2 -1样反应。此外，通过改变抗原Ig分子的分子形式，并指定靶受体，抗原可以被引导到MHC I类或MHC II类抗原递呈途径。本提案描述了建立抗体定向抗原呈递导致免疫的基本原理的实验。更重要的是，这些实验将导致新一代疫苗的设计，能够指导类别，从而提高免疫反应的有效性。

DESCRIPTION (provided by applicant): The process of immunization involves antigen uptake and processing by professional antigen presenting cells (APCs), activation of APCs, and migration of antigen-bearing cells to lymphoid organs. This in turn can result in the activation of T lymphocytes. This process is dependent upon the presence of inflammatory signals usually provided by microbial pattern recognition. Previous work has shown that antibody molecules provide an ideal delivery vehicle for antigen presentation and vaccination. Antibodies can efficiently direct an associated antigen to a particular cellular subset, such as dendritic cells, and simultaneously fulfill the requirement for cellular activation. Depending on the type of dendritic subset targeted, a prediction is that a Th1 or Th2-1ike responses will result. In addition, by altering the molecular form of the antigenic Ig molecules, and specifying a target receptor, antigen may be directed into either the MHC class I or MHC class II antigen presentation pathways. This proposal describes experiments to establish the basic principles of antibody-directed antigen presentation leading to immunization. More importantly, the experiments will lead to the design of a new generation of vaccines capable of directing the class and thus the effectiveness of immune responses.