Monoclonal antibodies for Lassa fever vaccine design and therapeutics

采用改进的血浆细胞igg克隆方法和抗原染色记忆B细胞富集方法分离新型拉沙热单克隆抗体。也将有机会利用噬菌体展示技术作为抗体分离的替代方法。虽然抗体克隆是这项工作的关键要素，但该项目的大部分工作将用于描述抗体的特征，包括表位定位、复制能力嵌合VSV分离物的开发及其在抗体逃逸中的应用。

Using new improved methods for plasma cell Ig-cloning and to investigate antigen-staining memory B cell enrichment to isolate novel monoclonal antibodies to Lassa fever. There will also be the opportunity to utilise phage display techniques as an alternative approach to antibody isolation. Whilst antibody cloning is a key element of the work, the majority of the project will be spent characterising the antibodies to include epitope mapping, development of replication-competent chimeric VSV isolates and their use in antibody escape.