Genetics of Programmed Cell Death in Arabidopsis

描述（由申请人提供）：植物，像所有高等真核生物，必须 控制细胞死亡的发生和扩散。植物生物学充满了 发育控制的程序性细胞死亡（POD）。 此外，植物中POD的一种特殊形式，称为过敏性 反应（HR）与成功识别密切相关， 对病原体攻击的反应因此，人力资源是潜在的重要组成部分， 植物的先天免疫反应然而，尚不清楚HR在其中的因果作用是什么。 在阻止病原体进入中起作用，或者HR是否是 植物的防御反应，杀死病原体。同样不清楚的是， HR的遗传控制是否在机制上与HR相关或不同， 从，机制控制植物PCD在发展过程中。此外，本发明还 虽然对动物中PCD控制的分子机制了解很多， 基本上对植物一无所知。 这项建议的重点是使用拟南芥作为遗传模型， 理解HR的控制是植物POD的一个容易定义的模型。 拟南芥基因组已完全测序， 动物POD关键调控因子在序列中的保守性证据 水平我们是最早认识到拟南芥可以用来 从基因上剖析细胞死亡控制， 到外地。我们鉴定并分析了一系列突变体 无病原体时HR样细胞死亡。我们克隆了三个关键的HR 所有调节子都属于一个基因家族： LSD 1和相关基因LOL 1（LSD One Like 1）和LOL 2。我们也 表明LSD1与两类转录因子相互作用， 这些蛋白质在HA中具有生物学功能，并且它们 在体内与LSD1相互作用。在此基础上，并在此基础上， 报告如下，具体目标的主题，使用前瞻性和 反向遗传学和生物化学是： 1.拟南芥LSD1蛋白：控制细胞死亡繁殖。 2. isdi信号转导：基因外isdi的遗传特征 抑制剂 3. LSD1的分子同源物也控制细胞死亡。 4. Isd 1 rcd与RO1、NO和SA平衡之间的相互作用。

DESCRIPTION (provided by applicant): Plants, like all higher eukaryotes, must control the onset and spread of cell death. Plant biology is replete with examples of developmentally controlled programmed cell death (POD). Additionally, a specialized form of POD in plants, termed the Hypersensitive Response (HR) is tightly correlated with successful recognition of, and response to, pathogen attack. Thus, the HR is potentially an important part of the plant innate immune response. It is, however, unclear what causal role HR plays in stopping pathogen ingress, or whether HR is the consequence of the plant defense response that does kill the pathogen. It is equally unclear whether the genetic control of HR is mechanistically related to, or distinct from, the mechanisms that control plant PCD during development. Additionally, while much is known about the molecular mechanisms of PCD control in animals, essentially nothing is known in plants. This proposal is focused on using Arabidopsis as a genetic model with which to understand the control of HR as an easily definable model for POD in plants. The Arabidopsis genome is fully sequenced and there is little molecular evidence for conservation of key regulators of animal POD at the sequence level. We were among the first to recognize the use of Arabidopsis to genetically dissect cell death control and have made significant contributions to the field. We identified and analyzed a series of mutants that misregulate HR-like cell death in the absence of pathogen. We cloned three key HR regulators all belonging to one gene family: LSD1 and the related genes LOL 1 (LSD One Like 1) and LOL2. We also demonstrated that LSD1 interacts with two classes of transcriptional regulators, that these proteins have biological functions in HA, and that they interact with LSD1 in viva. Based on this previous work, and on the progress reported below, the topics for the Specific Aims, using both forward and reverse genetics, and biochemistry, are: 1. The Arabidopsis LSD1 protein: Control of cell death propagation. 2. isdi signal transduction: Genetic characterization of extragenic isdi suppressors. 3. Molecular homologues of LSD1 also control cell death. 4. The interplay between Isd1 rcd and balances of ROl, NO and SA.