LC Microdispenser for High Resolution MALDI-TOF Analysis

描述（由申请人提供）：我们将构建一个LC微分点系统，用于快速液相色谱和高通量将消化的蛋白质样品装载到MALDI-TOF MS目标上。该系统将包括反相柱的自动制备，包括自动样品装载和集成到微点胶装置中。多流体打印头的容量将增加到20种不同的流体，以增加多个样品的吞吐量和/或允许不同的色谱处理。该系统将提供最大限度的样品体积保护，并结合快速LC分离肽，通过减少离子抑制，从而改善MALDI-TOF MS分析。通过对酿酒酵母菌低丰度蛋白的检测和肽质量指纹图谱，验证LC微分点系统的功能和灵敏度。商业化工作将与研究工作并行进行，与我们的商业化合作伙伴：Proteome Systems， Ltd.，岛津生物技术公司和密理博公司合作。

DESCRIPTION (provided by applicant): We will construct a LC Microdispenser system for rapid liquid chromatography and high throughput loading of digested protein samples onto MALDI-TOF MS targets. This system will incorporate automated preparation of the reversed phase column, including automatic sample loading and integration into the microdispensing device. The capacity of the multi-fluid print head will be increased to 20 different fluids to increase throughput for multiple samples and/or to allow for different chromatography treatments. This system will provide for the maximum conservation of sample volume combined with the rapid LC separation of peptides, resulting in improved MALDI-TOF MS analysis through a reduction in ion suppression. The function and sensitivity of the LC Microdispensor system will be verified by the detection and peptide mass fingerprinting of low abundance proteins in Saccharomyces cerevisiae. Commercialization efforts will be conducted in parallel with the research efforts, in collaboration with our commercialization partners: Proteome Systems, Ltd., Shimadzu Biotech, and Millipore Corporation. Commercial Applications: High throughput instrumentation that improves the sensitivity and dynamic range during MALDI-TOF MS and results in the peptide mass fingerprinting of low abundance proteins is highly desired in the rapidly developing field of proteomics. Details regarding protein expression and post-translational modifications can reveal processes within a biological system useful for drug discovery and the diagnosis and treatment of disease and cancer.