Optical Dosimetry in Laser-Assisted Cancer Immunotherapy

描述（由申请人提供）：这项工作涉及激光辅助免疫治疗的光学剂量学方面，这是一种新的癌症治疗方式。该疗法利用在激光照射前将光吸收染料和免疫佐剂共同施用到肿瘤体积中。将转移性乳腺肿瘤细胞植入Balb/C小鼠体内。肿瘤将在红外吸收染料和免疫佐剂的共同管理下用二极管激光照射。该项目的长期目标是确定能引起强烈肿瘤反应的轻剂量，以完全根除原发肿瘤和远处转移。激光照射会引起肿瘤的完全光热破坏，导致组织坏死。坏死程度是肿瘤温度的重要作用，它应该通过抗原呈递来决定肿瘤的反应。根据动态损伤模型对标准生物传热方程进行数值求解，得到预测肿瘤温度分布。为使肿瘤产生持久而有力的免疫反应而诱导最佳坏死的轻剂量将在实验中确定。

DESCRIPTION (provided by applicant): This work is concerned with the optical dosimetric aspect of Laser-Assisted Immunotherapy, a novel treatment modality for cancer therapy. The therapy utilizes co-administration of a light absorbing dye and an immunoadjuvant into the tumor volume prior to laser irradiation. Metastatic mammary tumor cells will be implanted in Balb/C mice. The tumor will be irradiated with a diode laser following the co-administration of an infrared absorbing dye and an immunoadjuvant. The long-term objective of this project is to determine the light dose which would invoke a strong tumor response for the total eradication of the primary tumor and distant metastases. A complete photo-thermal destruction of the tumor will be initiated with the laser irradiation, resulting in tissue necrosis. The extent of necrosis, which should determine the tumor response through antigen presentation, is a strong function of the tumor temperature. Numerical solution of the standard bioheat transfer equation in the light of a dynamic damage model will be obtained for the predicted temperature profile of the tumor. The light dose, which would induce an optimum necrosis for a sustained and vigorous immune response of the tumor, will be experimentally determined.