Polytene chromosomes of Culex pipiens mosquito complex

描述（由申请方提供）：尖音库蚊复合群的成员在世界范围内分布，在不同地区是圣路易斯脑炎、西尼罗河和裂谷热病毒以及班氏吴策线虫的媒介。复合物的两个成员，即Cx。p.pipiens和Cx.致倦库蚊（P.quinquefasciatus）的行为不一致，这与它们对疾病的媒介能力的可变性有关。这些亚种在吸血亲和力（哺乳动物与鸟类）、自生程度、休息行为（嗜内与嗜外）、病毒载体能力和交配行为方面各不相同。因此，有必要更明确地界定Cx成员的人口结构。pipiens复合体多线染色体重排可以作为一种工具，更清楚地表征和确定亚种内和亚种间的基因流动。本建议的总体目标是描述Cx成员的唾液腺多线染色体的特征。p.pipens complex.具体目的是：1）制作Cx唾腺染色体的照片图谱。p. pipiens，Cx. p.quinquefasciatus和Cx. 2）确定Cx. p. pipiens，Cx. p.quinquefasciatus和Cx.骚扰木

DESCRIPTION (provided by the applicant): Members of the Culex pipiens mosquito complex have a worldwide distribution and in various regions are vectors of St. Louis encephalitis, West Nile and Rift Valley fever viruses, and Wuchereria bancrofti. Two members of the complex, namely, Cx. p. pipiens and Cx. p. quinquefasciatus, are not uniform in their behavior, which is associated with variability in their vectorial capacities for diseases. These subspecies vary, in blood feeding avidity (mammal vs birds), degree of autogeny, resting behavior (endophilic vs. exophilic), vector competence for viruses and mating behavior. There is hence a need to more clearly define the population structure of members of the Cx. pipiens complex. Polytene chromosome rearrangements can serve as a tool to more clearly characterize and determine gene flow both within and between the subspecies. The overall objective of this proposal is to characterize the salivary gland polytene chromosomes of members of the Cx. p. pipens complex. The specific aims are: 1) Produce photomaps of salivary gland chromosomes of Cx. p. pipiens, Cx. p. quinquefasciatus and Cx. p. molestus and 2) Determine chromosomal homologies between Cx. p. pipiens, Cx. p. quinquefasciatus and Cx. p. molestus.