Phytochemical Treatments for Vocal Fold Scar

描述（由申请人提供）：医源性声带瘢痕导致严重的发音困难，难以成功治疗。透明质酸（HA）是存在于声带固有层细胞外基质中的重要分子，其有助于组织粘度以及声带振荡的启动和传播。旨在最大限度地提高伤口愈合过程中术后HA水平的技术可提供临床有用的方法，用于优化组织粘弹性，并最大限度地减少医源性瘢痕对声带生物力学特性的影响。松果菊苷、花青素和油酸是增加愈合伤口中HA沉积的植物化学物质。这些植物化学物质可以为疤痕提供有用的治疗。该提案旨在：1）量化声带瘢痕中的HA水平，2）评估植物化学处理对HA沉积的影响，和3）量化经处理和未经处理的组织样品的粘弹性剪切组织性质。组织学染色将用于测定HA，流变学方法将用于测量组织粘弹性。据推测，治疗的声带将显示出更多的HA沉积，并在术后表现出更小的硬度和动态粘度，这意味着植物化学治疗的组织中的粘弹性增强。

DESCRIPTION (provided by applicant): latrogenic vocal fold scarring results in a severe dysphonia, which is difficult to treat successfully. Hyaluronic acid (HA) is an important molecule found in the extracellular matrix of the vocal fold lamina propria, that contributes to tissue viscosity, and the initiation and propogation of vocal fold oscillation. Techniques aimed at maximizing post-operative HA levels during wound healing may offer clinically useful methods for optimizing tissue viscoelasticity, and minimizing the effects of iatrogenic scarring on vocal fold biomechanical properties. Echinacoside, Anthocyanoside, and Oleanolic Acid are phytochemicals that increase the deposition of HA in healing wounds. These phytochemicals may provide useful treatments for scarring. This proposal aims to: 1) Quantify HA levels in vocal fold scar, 2) Assess the effects of phytochemical treatments on deposition of HA, and 3) Quantify the viscoelastic shear tissue properties of treated and untreated tissue samples. Histologic staining will be used to assay HA, and rheologic methods will be used for measuring tissue viscoelasticity. It is hypothesized that the treated vocal fold will show greater deposition of HA, and present with less stiffness and dynamic viscosity post-operatively, signifying enhanced viscoelastic properties in phytochemically treated tissue.