Rapid Identification of Secreted of F tularensis Antigen

描述（由申请人提供）：细胞内细菌病原体在感染后分泌蛋白质，其功能是改变宿主细胞的正常结构和代谢机制，从而促进其生存并避免宿主免疫监视。最近发现的细胞内细菌病原体用来逃避或颠覆宿主免疫系统的分子机制将极大地促进抗菌疫苗和诊断工具的发展。就像许多其他细胞内兼性病原体，如鼠伤寒沙门氏菌、结核分枝杆菌和嗜肺军团菌一样，土拉菌也偏爱巨噬细胞作为其首选宿主细胞。然而，与其他细胞内细菌病原体相比，对土拉菌在其宿主细胞内使用的毒力因子知之甚少。我们设计并测试了一个系统，使我们能够识别鼠伤寒沙门氏菌中的I类可及蛋白（cap），并建议利用类似的方法研究土拉菌。由于它们对宿主的加工和呈递途径的易感性，cap为设计和构建有效的土拉菌病疫苗提供了潜在的重要资源。

DESCRIPTION (provided by applicant): Intracellular bacterial pathogens secrete proteins after infection that function to alter the normal structural and metabolic machinery of the host cell thus facilitating their survival and avoidance of host immune surveillance. Recent discoveries of the molecular mechanisms that intracellular bacterial pathogens use for evasion or subversion of the immune system of the host will greatly facilitate the development of antibacterial vaccines and diagnostic tools. Much like many other intracellular facultative pathogens such as Salmonella typhimurium, Mycobacterium tuberculosis and Legionella pneumophila, F. tularensis shares a predilection for macrophages as its preferred host cell. However, in contrast to other intracellular bacterial pathogens, little is known about virulence factors used by F. tularensis internally within its host cell. We have designed and tested a system that enables us to identify Class I Accessible Proteins (CAPs) in Salmonella typhimurium and propose to utilize a similar approach for the study of F. tularensis. Due to their susceptibility to the host' s processing and presentation pathways, CAPs represent a potentially important resource for the design and construction of effective vaccines against F. tularensis.