Apoptotic Cell Removal in Idiopathic Pulmonary Fibrosis

描述(由申请人提供)：这项研究计划旨在调查和确定特发性肺纤维化(IPF)患者中细胞凋亡的异常和凋亡细胞的去除。我们推测，细胞凋亡和凋亡细胞清除的异常导致转化生长因子-β1活性增加，并有助于本病肺纤维化的发展。这项提议有两个特定的目标：特定的目标1：定义IPF中的细胞凋亡和凋亡细胞清除的异常。特异目的2：探讨IPF中细胞凋亡、凋亡细胞清除与转化生长因子-β1产生的关系。人单核细胞来源的巨噬细胞、肺泡巨噬细胞和肺成纤维细胞将从IPF患者和年龄匹配的正常对照组获得。这些细胞的凋亡，以及这些细胞清除凋亡细胞的能力将被确定。IPF中细胞的凋亡和凋亡细胞的清除与转化生长因子-β1的产生程度有关。

DESCRIPTION (provided by applicant): This research proposal aims to investigate and define abnormalities in apoptosis and the removal of apoptotic cells in patients with Idiopathic Pulmonary Fibrosis (IPF). We hypothesize that abnormalities in apoptosis and apoptotic cell removal lead to increased TGF-beta 1 activity and contribute to the development of pulmonary fibrosis in this disease. There are two specific aims to this proposal: Specific Aim 1: To define abnormalities in apoptosis and apoptotic cell removal in IPF. Specific Aim 2: To determine the relationship between apoptosis, apoptotic cell removal and TGF-beta 1 production in IPF. Human monocyte derived macrophages, alveolar macrophages and lung fibroblasts will be obtained from subjects with IPF and normal age-matched controls. Apoptosis of these cells, and the ability of these cells to remove apoptotic cells will be determined. Apoptosis and apoptotic cell removal in IPF will be correlated with the degree of TGF-beta 1 production.