CORE--LARGE ANIMAL

该核心单位的目的是提供一个中心机制，以协助研究者设计和执行犬和非人灵长类动物的造血干细胞转导研究。核心股将提供三项主要职能。第一个功能，大型动物实验的设计和规划将涉及为个体研究提供犬和猴。核心单位负责人将确保动物可用于各项研究并进行充分准备。第二个功能涉及实际的干细胞收获和转导细胞的回输。骨髓干细胞将提供给个体研究者进行进一步处理和转导。在再输注转导细胞之前，动物将接受清髓剂量的全身照射。猴将接受静脉内导管，该导管通过系绳系统连接到笼外。这有助于液体和广谱抗生素的日常管理，也将用于转导细胞的再输注。第三个功能涉及移植后动物的监测和护理。所有动物将由核心部门负责人每日检查，并与灵长类动物中心和犬机构的经过专门培训的人员讨论健康状况和治疗计划。华盛顿大学的灵长类中心为我们的非人类灵长类研究留出了一个特殊的房间，以适应这些研究所需的重症监护。灵长类动物中心和犬科系的工作人员经过专门培训，可以与参与干细胞移植研究的动物一起工作。在许多方面，这个大型动物干细胞单位的运作是仿照人类干细胞移植单位，以确保这些研究所需的高质量护理。

The objective of this Core Unit is to provide a central mechanism to assist investigators with the design and execution of hematopoietic stem cell transduction studies in dogs and non-human primates. The Core Unit will provide three main functions. The first function, the design and the planning of the large animal experiments will involve the provision of dogs and monkeys for the individual studies. The Core Unit director will ensure that animals will be available and adequately prepared for the individual studies. The second function involves the actual stem cell harvest and the reinfusion of transduced cells. Marrow stem cells will be given to the individual investigators for further processing and transduction. Before reinfusion of transduced cells the animals will receive a myeloablative dose of total body irradiation. Monkeys will receive an intravenous catheter that is connected via a tether system to the outside of the cage. This facilitates the daily administration of fluids and broad-spectrum antibiotics and will also be used for reinfusion of transduced cells. The third function involves the post-transplant monitoring and care of the animals. All animals will be examined daily by the Core Unit director and health status and treatment plan will be discussed with specially trained personnel at the Primate Center and the Canine Facility. The Primate Center at the University of Washington has set aside a special room for our nonhuman primate studies to accommodate the intensive care required for these studies. The staff at the Primate Center and the Canine Faculty is specially trained to work with animals involved in stem cell transplantation studies. In many ways, the operations of this large animal stem cell unit is modeled after a human stem cell transplantation unit to assure the high quality care required for these studies.