CORE--MOUSE

小鼠核心的主要目标是提供一个集中的设施，以确保每个参与项目的实验室生产和维持转基因和基因破坏小鼠的能力。本提案中的所有四个项目都将在其研究中利用转基因方法。设立一个核心小组将可避免需要专门设备的高度技术性技能的重复，从而节省工作和费用。核心将促进项目之间的合作。已在Parnassus Campus获得了用于ES细胞培养和卵和囊胚显微注射的专用空间和设备，并为主要研究者分配了足够的动物空间以履行核心职能。虽然支持这个核心的请求是新的，但在过去的六个月里，我们一直在以非常有效的方式运营一个功能性虚拟核心。本提案的进度报告和初步数据部分中所述的大多数鼠标线都是使用下述人员和设施生成的。

The major objective of the Mouse Core is to provide a centralized facility to insure the capacity of each participating project lab to produce and maintain transgenic and gene-disrupted mice. All four projects in this proposal will utilize transgenic approaches in their studies. The creation of a Core will result in economy of effort and expense by avoiding duplication of a highly technical skill requiring specialized equipment. The Core will facilitate collaborations between projects. Dedicated space and equipment for ES cell culture and microinjection of eggs and blastocysts has been secured on the Parnassus Campus and adequate animal space is assigned to the Principal Investigator for Core functions. Although the request for support for this Core is new for this grant, we have been operating a functional virtual core in a highly effective manner for the last six months. Most of the mouse lines described in the Progress Report and Preliminary Data sections of the proposal have been generated using the staff and facilities described below.