CORE--Flow Cytometry Core

该核心设施将为拟建中心内的一些项目提供流式细胞分析和流式细胞分离方面的支持。目前有三台机器可供使用：两台台式分析机和一台高速8色10参数Moflo分选机，具有单细胞沉积单元和四向分选能力。视资金情况，今后可能再购买一台分拣机（FACSAria）。为了增加对这一有限资源的利用，最近雇用了第二名技术人员，以增加机器可供使用的小时数。要求为这名技术员提供三分之一的薪金支助。该机构将提供一小组滴定试剂，用于鼠、人和狒狒细胞的典型流式细胞术分析。还将提供培训、数据存档和数据评价。项目1（目标3和4）、项目2（目标1-3）以及技术开发部分项目2和3需要使用流动设施。这特别包括逆转录病毒感染后GFP标记的骨髓细胞的分选以及起始群体如人肺泡巨噬细胞和外周血的分选 巨噬细胞对于巨噬细胞和树突状细胞的凋亡检测、PMN功能测试以及来自B的淋巴结、脾和外周血样品的分析，特别需要FACS分析。感染炭疽病的狒狒

This core facility will provide support with regard to flow cytometric analysis and flow cytometric cell separation for a number of projects within the proposed center. Three machines are currently accessible: two bench-top analysis machines and one high-speed 8-color, 10-parameter Moflo sorter with single-cell deposition unit and four-way sorting capabilities. An additional sorter (FACSAria) might be purchased in the future depending on availability of funding. To increase access to this limited resource, a second technician was recently hired to increase the number of hours the machines are accessible for use. One-third salary support is requested for this technician. The facility will provide a small panel of titrated reagents for typical flow cytometric analysis of murine, human and baboon cells. Training, data archiving and data evaluation will also be provided. Projects 1 (Aims 3 and 4), Project 2 (Aims 1-3) as well as Technology Development Component projects 2 and 3 require access to flow facilities. This includes particularly the sorting of GFP-tagged myeloid cells after retroviral infection as well as sorting of starting populations, such as human alveolar macrophages and peripheral blood macrophages. FACS-analysis is required in particular for apoptosis detection of macrophages and dendritic cells, for PMN functional tests and for the analysis of lymph node, spleen and peripheral blood samples from B. anthracis-infected baboons.