BD FACSAria Desktop Cell Sorter

BD FACSAria 台式细胞分选仪

• 批准号: 6738504
• 负责人: Paul Wayne Kincade
• 金额: $ 35.2万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6738504
• 关键词: biomedical equipment purchase; flow cytometry

DESCRIPTION (provided by applicant): A BD FACSAria TM desktop cell sorter is being requested in this shared instrumentation proposal. It will be installed in an existing core facility that serves more than 40 investigators, who collectively have 49 NIH grants, in addition to support from NSF and private agencies. A very highly trained technician efficiently operates a Cytomation MoFIo cell sorter in this facility. However, the instrument is over-booked and cannot be used with less than two weeks advanced notice. Also, the complexity of the MoFIo is such that it cannot be operated by less skilled personnel. In contrast, the FACSAria is user friendly, does not require daily calibration and has nearly the same capability for high speed, high purity sorts. Moreover, it occupies a fraction of the space and utilizes low maintenance, air-cooled lasers. Therefore, this instrument will more than double our capacity for cell sorting, removing a very serious limitation to progress in many NIH supported projects. In addition, it will allow the facility to better serve our community by encouraging new research applications that depend on cell sorting. Management procedures for sorting will continue as before, but we will be able to resume training activities that were suspended for lack of capacity. Relevant data will be archived and transferred to investigators as is currently done with the existing cell sorter and two flow cytometers. All of these instruments will continue to be well maintained under full service contracts.

描述（由申请人提供）：本共享仪器提案中要求使用 BD FACSAria TM 台式细胞分选仪。它将安装在现有的核心设施中，该设施为 40 多名研究人员提供服务，除了 NSF 和私人机构的支持外，这些研究人员总共拥有 49 项 NIH 拨款。训练有素的技术人员在该设施中高效地操作 Cytomation MoFIo 细胞分选机。然而，该仪器已被超额预订，如果提前两周通知，则无法使用。此外，MoFIo 的复杂性使得它无法由不太熟练的人员操作。相比之下，FACSAria 用户友好，不需要日常校准，并且具有几乎相同的高速、高纯度分选能力。此外，它只占用一小部分空间，并采用低维护的风冷激光器。因此，该仪器将使我们的细胞分选能力增加一倍以上，消除许多 NIH 支持项目进展的严重限制。此外，它还将使该设施能够通过鼓励依赖细胞分选的新研究应用来更好地服务我们的社区。分类管理程序将像以前一样继续进行，但我们将能够恢复因缺乏能力而暂停的培训活动。相关数据将被存档并传输给研究人员，就像目前使用现有细胞分选仪和两台流式细胞仪所做的那样。所有这些仪器将根据全面服务合同继续得到良好维护。