BD FACSAria Desktop Cell Sorter

描述（由申请人提供）：在本共享仪器提案中要求使用BD FACSAria TM台式细胞分选仪。它将安装在现有的核心设施中，该设施为40多名研究人员提供服务，这些研究人员除了获得NSF和私人机构的支持外，还获得了49项NIH赠款。一名训练有素的技术人员在该设施中高效地操作Cytomation MoFIo细胞分选机。但是，该仪器已超额预订，提前两周通知后无法使用。此外，MoFIo的复杂性使其无法由技术水平较低的人员操作。相比之下，FACSAria是用户友好的，不需要每天校准，并具有几乎相同的能力，高速，高纯度的排序。此外，它占据了一小部分空间，并利用低维护，空气冷却激光器。因此，该仪器将使我们的细胞分选能力增加一倍以上，消除了许多NIH支持项目进展的严重限制。此外，它还将通过鼓励依赖细胞分选的新研究应用，使该设施更好地为我们的社区服务。分类的管理程序将一如既往，但我们将能够恢复因缺乏能力而暂停的培训活动。相关数据将被存档并转移给研究者，就像目前使用现有细胞分选仪和两台流式细胞仪所做的那样。根据全面服务合同，所有这些仪器将继续得到良好的维护。

DESCRIPTION (provided by applicant): A BD FACSAria TM desktop cell sorter is being requested in this shared instrumentation proposal. It will be installed in an existing core facility that serves more than 40 investigators, who collectively have 49 NIH grants, in addition to support from NSF and private agencies. A very highly trained technician efficiently operates a Cytomation MoFIo cell sorter in this facility. However, the instrument is over-booked and cannot be used with less than two weeks advanced notice. Also, the complexity of the MoFIo is such that it cannot be operated by less skilled personnel. In contrast, the FACSAria is user friendly, does not require daily calibration and has nearly the same capability for high speed, high purity sorts. Moreover, it occupies a fraction of the space and utilizes low maintenance, air-cooled lasers. Therefore, this instrument will more than double our capacity for cell sorting, removing a very serious limitation to progress in many NIH supported projects. In addition, it will allow the facility to better serve our community by encouraging new research applications that depend on cell sorting. Management procedures for sorting will continue as before, but we will be able to resume training activities that were suspended for lack of capacity. Relevant data will be archived and transferred to investigators as is currently done with the existing cell sorter and two flow cytometers. All of these instruments will continue to be well maintained under full service contracts.