BD FACSAria Desktop Cell Sorter

描述(由申请人提供)：BD FACSAria TM台式细胞分选机在此共享仪器提案中被请求。它将安装在一个现有的核心设施中，该设施服务于40多名调查人员，除了来自NSF和私人机构的支持外，他们总共拥有49项NIH拨款。一位训练有素的技术人员在这个设施中高效地操作细胞分裂MoFIo细胞分选机。然而，该仪器已被超额预订，提前不到两周的通知就不能使用。此外，MoFIo的复杂性使得它不能由技能较低的人员操作。相比之下，FACSAria是用户友好的，不需要日常校准，并具有几乎相同的能力，高速，高纯度的分类。此外，它只占用了一小部分空间，并使用了维护成本低的风冷激光器。因此，这种仪器将使我们的细胞分选能力增加一倍以上，消除了许多NIH支持的项目进展的非常严重的限制。此外，它还将通过鼓励依赖细胞分选的新研究应用，使该设施更好地服务于我们的社区。分类的管理程序将一如既往地继续，但我们将能够恢复因缺乏能力而暂停的培训活动。相关数据将被存档并移交给调查人员，就像目前使用现有的细胞分类器和两个流动细胞仪所做的那样。根据全面服务合同，所有这些文书将继续得到妥善维护。

DESCRIPTION (provided by applicant): A BD FACSAria TM desktop cell sorter is being requested in this shared instrumentation proposal. It will be installed in an existing core facility that serves more than 40 investigators, who collectively have 49 NIH grants, in addition to support from NSF and private agencies. A very highly trained technician efficiently operates a Cytomation MoFIo cell sorter in this facility. However, the instrument is over-booked and cannot be used with less than two weeks advanced notice. Also, the complexity of the MoFIo is such that it cannot be operated by less skilled personnel. In contrast, the FACSAria is user friendly, does not require daily calibration and has nearly the same capability for high speed, high purity sorts. Moreover, it occupies a fraction of the space and utilizes low maintenance, air-cooled lasers. Therefore, this instrument will more than double our capacity for cell sorting, removing a very serious limitation to progress in many NIH supported projects. In addition, it will allow the facility to better serve our community by encouraging new research applications that depend on cell sorting. Management procedures for sorting will continue as before, but we will be able to resume training activities that were suspended for lack of capacity. Relevant data will be archived and transferred to investigators as is currently done with the existing cell sorter and two flow cytometers. All of these instruments will continue to be well maintained under full service contracts.