BD FACSAria Desktop Cell Sorter

描述（由申请人提供）：在这个共享仪器提案中，需要BD FACSAria TM台式细胞分选仪。它将被安装在一个现有的核心设施中，该设施为40多名研究人员提供服务，这些研究人员共有49项NIH资助，此外还有来自NSF和私人机构的支持。一个非常训练有素的技术人员有效地操作在这个设施的细胞计数MoFIo细胞分选器。但是，该工具已超额预订，提前两周内不能使用。此外，MoFIo的复杂性使其无法由技能较差的人员操作。相比之下，FACSAria用户友好，不需要每天校准，并且具有几乎相同的高速，高纯度分类能力。此外，它占用空间的一小部分，并利用低维护，气冷激光器。因此，该仪器将使我们的细胞分选能力增加一倍以上，消除了许多NIH支持项目进展的一个非常严重的限制。此外，它将通过鼓励依赖于细胞分选的新研究应用，使该设施更好地服务于我们的社区。分类管理程序将一如既往地继续进行，但我们将能够恢复因能力不足而暂停的培训活动。相关数据将存档并转交给研究人员，就像目前使用现有的细胞分选器和两台流式细胞仪一样。根据全面服务合同，所有这些仪器将继续得到良好的维护。

DESCRIPTION (provided by applicant): A BD FACSAria TM desktop cell sorter is being requested in this shared instrumentation proposal. It will be installed in an existing core facility that serves more than 40 investigators, who collectively have 49 NIH grants, in addition to support from NSF and private agencies. A very highly trained technician efficiently operates a Cytomation MoFIo cell sorter in this facility. However, the instrument is over-booked and cannot be used with less than two weeks advanced notice. Also, the complexity of the MoFIo is such that it cannot be operated by less skilled personnel. In contrast, the FACSAria is user friendly, does not require daily calibration and has nearly the same capability for high speed, high purity sorts. Moreover, it occupies a fraction of the space and utilizes low maintenance, air-cooled lasers. Therefore, this instrument will more than double our capacity for cell sorting, removing a very serious limitation to progress in many NIH supported projects. In addition, it will allow the facility to better serve our community by encouraging new research applications that depend on cell sorting. Management procedures for sorting will continue as before, but we will be able to resume training activities that were suspended for lack of capacity. Relevant data will be archived and transferred to investigators as is currently done with the existing cell sorter and two flow cytometers. All of these instruments will continue to be well maintained under full service contracts.