Spectratyping Core

光谱分析是一种基于TCR受体利用率测量T细胞应答的快速方法。它包括基于它们表达的V基因以及被称为第三互补决定区CDR 3的抗原接触区的长度来分裂应答T细胞。该技术包括扩增对应于TCR链之一的mRNA的cDNA，并在变性凝胶上分离扩增产物。通常，正常组库由对应于不同CDR 3长度的多个条带表示，其强度遵循泊松分布。在用抗原刺激后，对应于来自对抗原应答的细胞的TCR的条带显示出增加的强度。 我们预计核心实验室每年将进行超过1000次频谱测试，以支持这三个项目，以及支持技术目标1和一些试点项目。通过集中这些分析，我们将允许各个项目负责人专注于他们正在进行的研究的其他方面。此外，核心将保证为中心的所有研究部门提供可复制的数据集。

Spectratyping is a rapid method for measuring T cell responses on the basis of TCR receptor utilization. It consists of dividing the responding T cell on the basis of the V gene they express as well as the length of the antigen contact region referred to as the third complementarity determining region, CDR3. The technique consists of amplifying the cDNA corresponding to mRNA for one of the TCR chains and separating the amplification product on denaturing gels. Typically, a normal repertoire is represented by multiple bands corresponding to different CDR3 lengths, whose intensity follows a Poisson distribution. Upon stimulation with antigen, bands corresponding to TCR from cells that responded to the antigen show increased intensity. We expect that the core laboratory will perform over 1000 spectratypes/year in support of these three projects as well as in support of technical Aim 1 and perhaps some of the pilot projects. By centralizing these analyses we will allow the individual project leaders to focus on other aspects of the research they are pursuing. In addition the Core will guarantee reproducible data sets for all the research components of the Center.