CORE--Microarray Core

本申请中描述的微阵列和生物信息学核心设施具有三部分集成结构。第一个方面涉及制作最先进的微阵列的技术能力;进行微阵列实验，包括RNA标记、阵列杂交和处理，然后对复杂基因数据集进行生物信息学分析。这里使用的阵列是在玻璃支持物上的长（70 mer）寡核苷酸阵列。使用荧光cDNA探针测定这些。第二部分是微阵列研究领域的适度技术发展。这涉及生物信息学和统计分析的技术优化和改进，并将采用众所周知的生物信息学微阵列数据库进行数据共享和数据存储。核心的最后一个组成部分是指导分子技术及其应用的研究人员。在应用程序中有一个固有的协同设计，用于与整个程序的其他领域和应用程序一起工作。

The Microarray and Bioinformatics Core Facility described in this application has a three part integrated structure. The first relates to the technical ability to produce state of the art microarrays; perform microarray experiments, including RNA labeling, array hybridization and processing, followed up by bioinformatics analysis of complex gene data sets. The arrays being used here are long (70 mer) oligonucleotide arrays on a glass support. These are assayed using fluorescent cDNA probes. The second part is modest technology development in the area of microarray research. This relates to technical optimization and improvements in bioinformatic and statistical analysis and will employ a well-known bioinformatics microarray database for data sharing and data storage. The last component of the core is mentoring researchers in molecular techniques and their applications. There is an inherent collaborative design in the application for working with other areas and applications of the overall program.