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CORE--Biochemical-Analysis Core

CORE--Biochemical-Analysis Core
CORE--生化分析核心
批准号：
6990371
负责人：
MYRON GOODMAN
金额：
$16.36万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
项目状态：
已结题
起止时间：
2004-08-16 至 2008-07-31
来源：
https://reporter.nih.gov/project-details/6990371
关键词：
biochemistrybiomedical facilitynucleic acid chemical synthesisprotein purification

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中文摘要
翻译
核心B是一个生化分析核心，将为所有三个项目提供不可或缺的服务。核心B的生化成分被设计用于进行蛋白质纯化和合成项目1和3所需的专门的DNA结构。分析部分将负责获取动力学数据并对其进行全面分析，包括用于支持项目2和3的稳态前停流荧光强度和旋转各向异性以及快速淬灭实验。核心B将作为培养研究生和博士后学生在蛋白质纯化技术和酶动力学分析的原理和实践的主要工具。
英文摘要
Core B is a biochemical analysis core that will provide indispensable services for all three projects. The biochemical component of Core B is designed to carry out protein purifications and to synthesize specialized DNA constructs required for Projects 1 and 3. The analysis component will be responsible for acquiring and performing global analysis on kinetic data, including presteady state stopped-flow fluorescence intensity and rotational anisotropy and rapid quench experiments, to be used in support of Projects 2 and 3. Core B will serve as a primary vehicle for training graduate and postdoctoral students in protein purification techniques and in the principles and practice of enzyme kinetic analysis.
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