High Productivity Eukaryotic Cell Culture Technology

描述（由申请人提供）：本项目的目标是开发一种方法，以增加真核细胞的生产和细胞质量的研究和药物发现。我们有初步的数据表明，我们已经开发出一种基于中性浮力水凝胶的微载体工艺，可以显着提高研究人员可用细胞的密度，活力和多样性，同时处理有限的空间并提高生产力需求。我们的新的基于微载体的细胞培养方法允许将锚定依赖性细胞从生长培养基直接转移到分析过程中，而不需要使用胰蛋白酶（或其他手段）从其锚定表面去除细胞。我们的微载体是光学透明的，不显示任何自发荧光，因此它们是基于光的显微镜和体外荧光研究的理想选择。我们已经证明，微载体的密度可以通过添加玻璃泡和顺磁性颗粒来操纵，以允许悬浮液生长而不使用叶轮。非接触式磁操作允许整个细胞生长过程与实验室自动化一起使用。 根据我们的初步数据，该提案主要围绕三个具体目标： 1.我们将改进我们的初步方法，用于生产中性浮力微载体，这些微载体针对各种前列腺癌和膀胱癌细胞的生长进行了优化，以便使癌症中心的研究科学家可以使用这些微载体，以获得用户反馈。 2.我们将在我们的新型微载体上培养细胞，以测量细胞活力、密度和形态。 3.我们将根据我们在实验室自动化和细胞培养方面多年的经验，为自动化细胞培养系统的设计开发一个概念框架。 我们的新型中性密度微载体将为研究科学家提高真核细胞的生产率，质量和可用性。

DESCRIPTION (provided by applicant): The goals of this project are to develop a method to increase eukaryotic cell production and cell quality for research and drug discovery. We have preliminary data that demonstrates that we have developed a neutral buoyancy hydrogel based microcarrier process that can dramatically increase the density, viability, and diversity of cells available to researchers while dealing with their limited space and increase productivity needs. Our novel microcarrier based cell culture process allows anchorage dependent cells to be transferred from the growth media directly into an analytical process without the need to use trypsin (or other means) to remove cells from their anchorage surface. Our microcarriers are optically clear and do not exhibit any autofluorescence, thus they are ideal for light based microscopy and in vitro fluorescence studies. We have demonstrated that the density of the microcarriers can be manipulated though the addition of glass bubbles and paramagnetic particles to allow suspension growth without the use of impellers. Non-contact magnetic manipulation permits the entire cell growth process to be used with laboratory automation. Given our preliminary data, this proposal is centered on three specific aims: 1. We will refine our preliminary method for producing neutral buoyancy microcarriers that are optimized for the growth of a variety of prostate cancer and bladder cancer cells in order to make them microcarriers available to cancer center research scientists for user feedback. 2. We will culture cells on our novel microcarriers in order to measure cell viability, density, and morphology. 3. We will develop a conceptual framework for the design of an automated cell culture system based on our many years of experience in laboratory automation and cell culture. Our novel neutral density microcarriers will improve the productivity, quality, and availability of eukaryotic cells for research scientists.